Phylogeographical break and limited connectivity between multiple refugia in panantarctic moss species ...

• Main Authors: Saługa, Marta, Ochyra, Ryszard, Ronikier, Michał
• Format: Dataset
• Language: English
• Published: Dryad 2022
• Subjects: FOS Biological sciences; Syntrichia sarconeurum; Syntrichia lithophila; Sanger sequencing; Antarctica; Phylogeography; Antarctic; Indel’; Patagonia; The Antarctic; Antarc*
• Online Access: https://dx.doi.org/10.5061/dryad.h18931zp3; https://datadryad.org/stash/dataset/doi:10.5061/dryad.h18931zp3

Aim: Historical biogeography of the Antarctic terrestrial biota remains poorly studied and understood. We aim to advance this through a range-wide, multilocus analysis of a pan-Antarctic moss species, in the context of its age, range dynamics, phylogeographical structure, and location of possible long-term refugia. Location: Continental and maritime Antarctic, South America (Patagonia), Australasia. Taxon: Syntrichia sarconeurum Ochyra & R.H. Zander (Pottiaceae). Methods: We used a comprehensive, range-wide and taxonomically-informed sampling, and multilocus sequencing of nuclear and plastid DNA regions. Temporal evolutionary framework, regional genetic diversification and diversity were assessed with phylogenetic and phylogeographic reconstructions, molecular dating, haplotype networks, mismatch analysis, and S-DIVA reconstruction of past events and ancestral areas. Results: Intercontinental disjunction between Australia and Antarctica/S. America was dated to 3.77 Ma, while diversification of extant ... : Plant sampling was based on collections gathered during Antarctic expeditions between 1940 and 2016, with specimens deposited in the bryological herbarium of the Institute of Botany, Polish Academy of Sciences (KRAM). One nuclear ribosomal marker, ITS1-5.8S-ITS2, and three plastid (cpDNA) markers, rps4, tRNAGly, and trnL-F, were selected for the multilocus analysis. Forward and reverse sequences were automatically assembled using Geneious 10.1.3. Sequences of the four DNA regions were individually aligned using the algorithm mafft-auto with the remaining default settings in Mafft (https://mafft.cbrc.jp/alignment/server//). Selected DNA regions were concatenated using SequenceMatrix. For all molecular analyses, indels in DNA sequences were coded and calculated in SeqState v1.4.1 using a simple indel coding. All programs are listed in the ReadMe text file. ...