Geography, seasonality, and host‐associated population structure influence the fecal microbiome of a genetically depauparate Arctic mammal ...

• Main Authors: Bird, Samantha, Prewer, Erin, Kutz, Susan, Leclerc, Lisa-Marie, Vilaça, Sibelle T., Kyle, Christopher J.
• Format: Dataset
• Language: English
• Published: Dryad 2020
• Subjects: 16S rRNA amplicon sequencing; Canadian Arctic; fecal microbiome; muskox; Ovibos moschatus; Arctic; Northwest Territories; Nunavut; ovibos moschatus; Victoria Island
• Online Access: https://dx.doi.org/10.5061/dryad.fj6q573q2; https://datadryad.org/stash/dataset/doi:10.5061/dryad.fj6q573q2

The Canadian Arctic is an extreme environment with low floral and faunal diversity characterized by major seasonal shifts in temperature, moisture and daylight. Muskoxen (Ovibos moschatus) are one of few large herbivores able to survive this harsh environment. Microbiome research of the gastrointestinal tract may hold clues as to how muskoxen exist in the Arctic, but also how this species may respond to rapid environmental changes. In this study, we investigated the effects of season (spring/summer/winter), year (2007-2016), and host genetic structure on population-level microbiome variation in muskoxen from the Canadian Arctic. We utilized 16S rRNA gene sequencing to characterize the fecal microbial communities of 78 male muskoxen encompassing two population genetic clusters. These clusters are defined by Arctic Mainland and Island populations, including; 1) two mainland sampling locations of the Northwest Territories and Nunavut; and 2) four locations of Victoria Island. Between these geographic ... : DNA from fecal samples were extracted using the Qiagen Power Fecal DNA extraction kit. The Illumina 16S metagenomic library protocol was followed. ...