Data from: The Genome sequence of a widespread apex predator, the golden eagle (Aquila chrysaetos) ...

Biologists routinely use molecular markers to identify conservation units, to quantify genetic connectivity, to estimate population sizes, and to identify targets of selection. Many imperiled eagle populations require such efforts and would benefit from enhanced genomic resources. We sequenced, asse...

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Bibliographic Details
Main Authors: Doyle, Jacqueline M., Katzner, Todd E., Bloom, Peter H., Ji, Yanzhu, Wijayawardena, Bhagya K., DeWoody, J. Andrew
Format: Dataset
Language:English
Published: Dryad 2015
Subjects:
avian genome
annotated genes
raptor
eagle conservation
genes
mitochondrial genome assembly
genetic markers
Transposable elements
protein sequences
Mira
Aquila chrysaetos
golden eagle
Online Access:https://dx.doi.org/10.5061/dryad.d0md6
https://datadryad.org/stash/dataset/doi:10.5061/dryad.d0md6
Description
Summary:Biologists routinely use molecular markers to identify conservation units, to quantify genetic connectivity, to estimate population sizes, and to identify targets of selection. Many imperiled eagle populations require such efforts and would benefit from enhanced genomic resources. We sequenced, assembled, and annotated the first eagle genome using DNA from a male golden eagle (Aquila chrysaetos) captured in western North America. We constructed genomic libraries that were sequenced using Illumina technology and assembled the high-quality data to a depth of ~40x coverage. The genome assembly includes 2,552 scaffolds >10 Kb and 415 scaffolds >1.2 Mb. We annotated 16,571 genes that are involved in myriad biological processes, including such disparate traits as beak formation and color vision. We also identified repetitive regions spanning 92 Mb (~6% of the assembly), including LINES, SINES, LTR-RTs and DNA transposons. The mitochondrial genome encompasses 17,332 bp and is ~91% identical to the Mountain ... : annotated genes_proteins16,571 genes were annotated in the golden eagle genome, this fasta file describes the protein sequences (see readme.pdf for more information). Corresponding file archived in fortress is kmer70_min10000_scaffolds_revisedassembly.all.maker.proteins.kmer70_min10000_scaffolds_revisedassembly.all.maker.proteins.fastaannotated genes_transcripts16,571 genes were annotated in the golden eagle genome, this fasta files describes the transcripts (see readme.pdf for additional information). Corresponding file archived in fortress is kmer70_min10000_scaffolds_revisedassembly.all.maker.transcripts.kmer70_min10000_scaffolds_revisedassembly.all.maker.transcripts.fastamitochondrial genome_GE_MITObim_revisedGolden eagle mitochondrial genome sequence assembled with MITObim vs. 1.6 and MIRA MIRA 3.4.1.1.GE_MITObim_revised.fastaxenobiotic sequences_Golden_Eagle_BLASTN_outputblastn (DiaGrid) Input Options Query File: GoldenEagle sequences with no significant match (E<10-6) to chicken genome Database ...

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