Uterine scarring leads to adverse pregnant consequence through impairing the response of endometrium to steroids ...

• Main Authors: Li, Zhilang, Bian, Xiaotao, Ma, Yeling, Yang, Qian, Jia, Wentong, Liu, Juan, Wang, Feiyang, Liu, Ming, Li, Yu-Xia, Shao, Xuan, Wang, Yan-Ling
• Format: Dataset
• Language: English
• Published: Dryad 2020
• Subjects: FOS Biological sciences; SCAR
• Online Access: https://dx.doi.org/10.5061/dryad.b8gtht790; https://datadryad.org/stash/dataset/doi:10.5061/dryad.b8gtht790

Uterine surgical scarring is an increasing risk factor for adverse pregnant consequences that threaten fetal-maternal health. The detailed molecular features of scar implantation remain largely unknown. We aim to study the pathologic features of uterine surgical scarring and the mechanisms of compromised pregnancy outcomes of scar implantation. We generated a mouse model of uterine surgical scarring with a uterine incision penetrating the myometrium to endometrium to examine the pathologic changes and transcriptome profiles of uterine scarring at various post-surgery (PS) time points, as well as features of the feto-maternal interface during scar implantation. We found that uterine surgical scar recovery was consistently poor at PS3 until PS90, as shown by a reduced number of endometrial glands, inhibition of myometrial smooth muscle cell growth but excessive collagen fiber deposition, and massive leukocyte infiltration. Transcriptome annotation indicated significant chronic inflammation at the scarring ... : Materials and methods Mouse model of uterine surgical scar Animal experiments were approved by the Experimental Animal Welfare Committee in the Institute of Zoology, Chinese Academy of Sciences. Female virgin CD1 mice (8 weeks) were purchased from Beijing SiPeiFu Animal Center (China) and housed at 25℃ with 12/12 hours day–night light cycles. Uterine surgery was performed under sterile conditions. Briefly, the mice were anaesthetized by intraperitoneal injection of 2% 2,2,2-Tribromoethanol (100ul/10g body weight), and a low abdominal incision was made to expose uterine horns. Under stereoscope, a 1cm-in-length longitudinal incision at the mid-portion of anti-mesometrial side of uterine horn was made by using ophthalmic scissors. The incision penetrated through myometrium and endometrium of anti-mesometrial side, but not harmed tissue of mesometrial side (Supplementary Figure S1A (13)). The incision was closed with 8-0 absorbable sutures using one-layer closure technique with interrupted lock stitches. To ...