Data from: Development, validation, and evaluation of an assay for the detection of wood frogs (Rana sylvatica) in environmental DNA ...
We developed and describe a qPCR assay for the detection of wood frogs (Rana sylvatica) using environmental DNA (eDNA) sampling. A single primer set was designed to amplify a 115-bp region of the wood frog cytochrome B gene and assessed for target specificity. There was no evidence of amplification...
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Online Access: | https://dx.doi.org/10.5061/dryad.b7g24 https://datadryad.org/stash/dataset/doi:10.5061/dryad.b7g24 |
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ftdatacite:10.5061/dryad.b7g24 2024-02-04T10:03:05+01:00 Data from: Development, validation, and evaluation of an assay for the detection of wood frogs (Rana sylvatica) in environmental DNA ... Spangler, Mark A. Huettmann, Falk Herriott, Ian C. López, J. Andrés 2018 https://dx.doi.org/10.5061/dryad.b7g24 https://datadryad.org/stash/dataset/doi:10.5061/dryad.b7g24 en eng Dryad https://dx.doi.org/10.1007/s12686-017-0881-3 Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 Rana sylvatica wood frog cytochrome b Dataset dataset 2018 ftdatacite https://doi.org/10.5061/dryad.b7g2410.1007/s12686-017-0881-3 2024-01-05T01:14:15Z We developed and describe a qPCR assay for the detection of wood frogs (Rana sylvatica) using environmental DNA (eDNA) sampling. A single primer set was designed to amplify a 115-bp region of the wood frog cytochrome B gene and assessed for target specificity. There was no evidence of amplification in 11 non-target species. We evaluated the utility of the primer set in qPCR assay by conducting geo-referenced eDNA field surveys in Interior Alaska. Results indicate that the assay consistently detects wood frog DNA in the environment to 1.83 × 10−3 pg/μL. The assay provides a complement to traditional survey methods and can be readily applied in a wider conservation and management context. ... : Wood Frog eDNA Survey Results1 liter water samples collected around Fairbanks/North Pole for eDNA analysis. Filters were cut in half and DNA isolated via phenol-chloroform from one half. Other half is archived. qPCR (SYBR Green) conducted for detection of wood frog cytB (Rasy_00)..Spangler_eDNA2015.csv ... Dataset North Pole Alaska DataCite Metadata Store (German National Library of Science and Technology) Fairbanks North Pole |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Rana sylvatica wood frog cytochrome b |
spellingShingle |
Rana sylvatica wood frog cytochrome b Spangler, Mark A. Huettmann, Falk Herriott, Ian C. López, J. Andrés Data from: Development, validation, and evaluation of an assay for the detection of wood frogs (Rana sylvatica) in environmental DNA ... |
topic_facet |
Rana sylvatica wood frog cytochrome b |
description |
We developed and describe a qPCR assay for the detection of wood frogs (Rana sylvatica) using environmental DNA (eDNA) sampling. A single primer set was designed to amplify a 115-bp region of the wood frog cytochrome B gene and assessed for target specificity. There was no evidence of amplification in 11 non-target species. We evaluated the utility of the primer set in qPCR assay by conducting geo-referenced eDNA field surveys in Interior Alaska. Results indicate that the assay consistently detects wood frog DNA in the environment to 1.83 × 10−3 pg/μL. The assay provides a complement to traditional survey methods and can be readily applied in a wider conservation and management context. ... : Wood Frog eDNA Survey Results1 liter water samples collected around Fairbanks/North Pole for eDNA analysis. Filters were cut in half and DNA isolated via phenol-chloroform from one half. Other half is archived. qPCR (SYBR Green) conducted for detection of wood frog cytB (Rasy_00)..Spangler_eDNA2015.csv ... |
format |
Dataset |
author |
Spangler, Mark A. Huettmann, Falk Herriott, Ian C. López, J. Andrés |
author_facet |
Spangler, Mark A. Huettmann, Falk Herriott, Ian C. López, J. Andrés |
author_sort |
Spangler, Mark A. |
title |
Data from: Development, validation, and evaluation of an assay for the detection of wood frogs (Rana sylvatica) in environmental DNA ... |
title_short |
Data from: Development, validation, and evaluation of an assay for the detection of wood frogs (Rana sylvatica) in environmental DNA ... |
title_full |
Data from: Development, validation, and evaluation of an assay for the detection of wood frogs (Rana sylvatica) in environmental DNA ... |
title_fullStr |
Data from: Development, validation, and evaluation of an assay for the detection of wood frogs (Rana sylvatica) in environmental DNA ... |
title_full_unstemmed |
Data from: Development, validation, and evaluation of an assay for the detection of wood frogs (Rana sylvatica) in environmental DNA ... |
title_sort |
data from: development, validation, and evaluation of an assay for the detection of wood frogs (rana sylvatica) in environmental dna ... |
publisher |
Dryad |
publishDate |
2018 |
url |
https://dx.doi.org/10.5061/dryad.b7g24 https://datadryad.org/stash/dataset/doi:10.5061/dryad.b7g24 |
geographic |
Fairbanks North Pole |
geographic_facet |
Fairbanks North Pole |
genre |
North Pole Alaska |
genre_facet |
North Pole Alaska |
op_relation |
https://dx.doi.org/10.1007/s12686-017-0881-3 |
op_rights |
Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 |
op_doi |
https://doi.org/10.5061/dryad.b7g2410.1007/s12686-017-0881-3 |
_version_ |
1789970254930116608 |