Data from: Tissue culture as a source of replicates in non-model plants: variation in cold response in Arabidopsis lyrata ssp. petraea ...
Whilst genotype-environment interaction is increasingly receiving attention by ecologists and evolutionary biologists, such studies need genetically homogeneous replicates-a challenging hurdle in outcrossing plants. This could potentially be overcome by using tissue culture techniques. However, plan...
| Main Authors: | , , , , , , |
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| Format: | Dataset |
| Language: | English |
| Published: |
Dryad
2017
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.5061/dryad.9gs8k https://datadryad.org/stash/dataset/doi:10.5061/dryad.9gs8k |
| Summary: | Whilst genotype-environment interaction is increasingly receiving attention by ecologists and evolutionary biologists, such studies need genetically homogeneous replicates-a challenging hurdle in outcrossing plants. This could potentially be overcome by using tissue culture techniques. However, plants regenerated from tissue culture may show aberrant phenotypes and "somaclonal" variation. Here we examined the somaclonal variation due to tissue culturing using the response to cold treatment of the photosynthetic efficiency (chlorophyll fluorescence measurements for Fv/Fm, Fv'/Fm' and ΦPSII, representing maximum efficiency of photosynthesis for dark- and light-adapted leaves, and the actual electron transport operating efficiency, respectively, which are reliable indicators of photoinhibition and damage to the photosynthetic electron transport system). We compared this to variation among half-sibling seedlings from three different families of Arabidopsis lyrata ssp. petraea. Somaclonal variation was limited ... : Data for Alp clone cold reaction for Dryad.xlsxThe row data is in "data" sheet. The description of the data is in "read.me" sheet. The description of the data: 1 row indicates 1 plant for which chlorophyll fluorescence was measured. Column "source". Which plants were derived from "seed" (i.e. seedling) or "clone" (i.e. tissule cultured plantlet). Column "w.genotype". If clone, name of genotype is provided. Otherwise, "half-sib". Column "pop". Name of populations. Either "ardal" in Norway, "notsand" in Sweden, or "sandfell" in Iceland. Column "treat". Experimental treatments. "pre": pre-cold treatment. "post": post-cold treatment. Column "ind". Number of plants. Column "block". Name of blocks. Chlorophyll fluorescence of plants in the same block was measured at the same time. Column "fvfm". Fv/Fm, chlorophyll fluorescence. Column "phy". Fv’/Fm’, chlorophyll fluorescence. Column "fvfm2". ФPSII, chlorophyll fluorescence. ... |
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