Supplementary information provided with Murray et al.: Discovery of an Antarctic ascidian-associated uncultivated Verrucomicrobia with antimelanoma palmerolide biosynthetic potential ...
The Antarctic marine ecosystem harbors a wealth of biological and chemical innovation that has risen in concert over millennia since the isolation of the continent and formation of the Antarctic circumpolar current. Scientific inquiry into the novelty of marine natural products produced by Antarctic...
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Online Access: | https://dx.doi.org/10.5061/dryad.8sf7m0cpp https://datadryad.org/stash/dataset/doi:10.5061/dryad.8sf7m0cpp |
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ftdatacite:10.5061/dryad.8sf7m0cpp 2024-02-04T09:56:03+01:00 Supplementary information provided with Murray et al.: Discovery of an Antarctic ascidian-associated uncultivated Verrucomicrobia with antimelanoma palmerolide biosynthetic potential ... Murray, Alison E Lo, Chien-Chi Daligault, Hajnalka E Avalon, Nicole E Read, Robert W Davenport, Karen W Higham, Mary L Kunde, Yuliya Dichosa, Armand EK Baker, Bill J Chain, Patrick SG 2021 https://dx.doi.org/10.5061/dryad.8sf7m0cpp https://datadryad.org/stash/dataset/doi:10.5061/dryad.8sf7m0cpp en eng Dryad https://dx.doi.org/10.1101/2021.05.05.442870 https://dx.doi.org/10.5281/zenodo.5586111 Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 FOS Biological sciences Dataset dataset 2021 ftdatacite https://doi.org/10.5061/dryad.8sf7m0cpp10.1101/2021.05.05.44287010.5281/zenodo.5586111 2024-01-05T04:39:59Z The Antarctic marine ecosystem harbors a wealth of biological and chemical innovation that has risen in concert over millennia since the isolation of the continent and formation of the Antarctic circumpolar current. Scientific inquiry into the novelty of marine natural products produced by Antarctic benthic invertebrates led to the discovery of a bioactive macrolide, palmerolide A, that has specific activity against melanoma and holds considerable promise as an anticancer therapeutic. While this compound was isolated from the Antarctic ascidian Synoicum adareanum, its biosynthesis has since been hypothesized to be microbially mediated, given structural similarities to microbially-produced hybrid non-ribosomal peptide-polyketide macrolides. Here, we describe a metagenome-enabled investigation aimed at identifying the biosynthetic gene cluster (BGC) and palmerolide A-producing organism. A 74 Kbp candidate BGC encoding the multi-modular enzymatic machinery (hybrid Type I-trans-AT polyketide ... : See the publication for details (main text and supplemental methods section). Briefly, we used a metagenomic approach to sequence a microbial community from an Antarctic ascidian, Synoicum adareanum. From this we assembled a nearly complete metagenome assembled genome (MAG) that encodes a candidate palmerolide A biosynthetic gene cluster of a verrucomicrobium affiliated new bacterium named in this manuscript as Candidatus Synoicihabitans palmerolidicus. The supplemental material provided in this data set includes (i) Figure S1 which provides correlation coefficients derived between real time PCR data (methods described in the manuscript) and amplicon sequence variant (ASV) occurrences across a data set of 63 samples. (ii) Figure S2 displays the taxonomic classification of predicted proteins in the assembled MAG derived from an annotation pipeline called MetaERG. (iii) The annotation of the Ca. S. palmerolidicus genome, as a GFF file formatted file. ... Dataset Antarc* Antarctic DataCite Metadata Store (German National Library of Science and Technology) Antarctic The Antarctic |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
FOS Biological sciences |
spellingShingle |
FOS Biological sciences Murray, Alison E Lo, Chien-Chi Daligault, Hajnalka E Avalon, Nicole E Read, Robert W Davenport, Karen W Higham, Mary L Kunde, Yuliya Dichosa, Armand EK Baker, Bill J Chain, Patrick SG Supplementary information provided with Murray et al.: Discovery of an Antarctic ascidian-associated uncultivated Verrucomicrobia with antimelanoma palmerolide biosynthetic potential ... |
topic_facet |
FOS Biological sciences |
description |
The Antarctic marine ecosystem harbors a wealth of biological and chemical innovation that has risen in concert over millennia since the isolation of the continent and formation of the Antarctic circumpolar current. Scientific inquiry into the novelty of marine natural products produced by Antarctic benthic invertebrates led to the discovery of a bioactive macrolide, palmerolide A, that has specific activity against melanoma and holds considerable promise as an anticancer therapeutic. While this compound was isolated from the Antarctic ascidian Synoicum adareanum, its biosynthesis has since been hypothesized to be microbially mediated, given structural similarities to microbially-produced hybrid non-ribosomal peptide-polyketide macrolides. Here, we describe a metagenome-enabled investigation aimed at identifying the biosynthetic gene cluster (BGC) and palmerolide A-producing organism. A 74 Kbp candidate BGC encoding the multi-modular enzymatic machinery (hybrid Type I-trans-AT polyketide ... : See the publication for details (main text and supplemental methods section). Briefly, we used a metagenomic approach to sequence a microbial community from an Antarctic ascidian, Synoicum adareanum. From this we assembled a nearly complete metagenome assembled genome (MAG) that encodes a candidate palmerolide A biosynthetic gene cluster of a verrucomicrobium affiliated new bacterium named in this manuscript as Candidatus Synoicihabitans palmerolidicus. The supplemental material provided in this data set includes (i) Figure S1 which provides correlation coefficients derived between real time PCR data (methods described in the manuscript) and amplicon sequence variant (ASV) occurrences across a data set of 63 samples. (ii) Figure S2 displays the taxonomic classification of predicted proteins in the assembled MAG derived from an annotation pipeline called MetaERG. (iii) The annotation of the Ca. S. palmerolidicus genome, as a GFF file formatted file. ... |
format |
Dataset |
author |
Murray, Alison E Lo, Chien-Chi Daligault, Hajnalka E Avalon, Nicole E Read, Robert W Davenport, Karen W Higham, Mary L Kunde, Yuliya Dichosa, Armand EK Baker, Bill J Chain, Patrick SG |
author_facet |
Murray, Alison E Lo, Chien-Chi Daligault, Hajnalka E Avalon, Nicole E Read, Robert W Davenport, Karen W Higham, Mary L Kunde, Yuliya Dichosa, Armand EK Baker, Bill J Chain, Patrick SG |
author_sort |
Murray, Alison E |
title |
Supplementary information provided with Murray et al.: Discovery of an Antarctic ascidian-associated uncultivated Verrucomicrobia with antimelanoma palmerolide biosynthetic potential ... |
title_short |
Supplementary information provided with Murray et al.: Discovery of an Antarctic ascidian-associated uncultivated Verrucomicrobia with antimelanoma palmerolide biosynthetic potential ... |
title_full |
Supplementary information provided with Murray et al.: Discovery of an Antarctic ascidian-associated uncultivated Verrucomicrobia with antimelanoma palmerolide biosynthetic potential ... |
title_fullStr |
Supplementary information provided with Murray et al.: Discovery of an Antarctic ascidian-associated uncultivated Verrucomicrobia with antimelanoma palmerolide biosynthetic potential ... |
title_full_unstemmed |
Supplementary information provided with Murray et al.: Discovery of an Antarctic ascidian-associated uncultivated Verrucomicrobia with antimelanoma palmerolide biosynthetic potential ... |
title_sort |
supplementary information provided with murray et al.: discovery of an antarctic ascidian-associated uncultivated verrucomicrobia with antimelanoma palmerolide biosynthetic potential ... |
publisher |
Dryad |
publishDate |
2021 |
url |
https://dx.doi.org/10.5061/dryad.8sf7m0cpp https://datadryad.org/stash/dataset/doi:10.5061/dryad.8sf7m0cpp |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
https://dx.doi.org/10.1101/2021.05.05.442870 https://dx.doi.org/10.5281/zenodo.5586111 |
op_rights |
Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 |
op_doi |
https://doi.org/10.5061/dryad.8sf7m0cpp10.1101/2021.05.05.44287010.5281/zenodo.5586111 |
_version_ |
1789960411342176256 |