Data from: A mobile laboratory for ancient DNA analysis ...

• Main Authors: Utge, Jose, Elalouf, Jean-Marc, Sévêque, Noémi, Lartigot-Campin, Anne-Sophie, Testu, Agnès, Moigne, Anne-Marie, Vézian, Régis, Maksud, Frédéric, Begouën, Robert, Soriano, Sylvain, Verna, Chistine
• Format: Dataset
• Language: English
• Published: Dryad 2019
• Subjects: Bison Priscus; Bison schoetensacki; Rangifer tarandus; NGS libraries
• Online Access: https://dx.doi.org/10.5061/dryad.8gtht76jz; https://datadryad.org/stash/dataset/doi:10.5061/dryad.8gtht76jz

Mobile devices for on-field DNA analysis have been used for medical diagnostics at the point-of-care, forensic investigation and environmental survey, but still have to be validated for ancient DNA studies. We report here on a mobile laboratory that we setup using commercially available devices, including a compact real-time PCR machine, and describe procedures to perform DNA extraction and analysis from a variety of archeological samples within 4 hours. The process is carried out on 50 mg samples that are identified at the species level using custom TaqMan real-time PCR assays for mitochondrial DNA fragments. We evaluated the potential of this approach in museums lacking facilities for DNA studies by analyzing samples from the Enlène (MIS 2 layer) and the Western Portel cave (MIS 3 deposits), and also performed experiments during an excavation campaign at the Roc-en-Pail (MIS 5) open-air site. Enlène Bovinae bone samples only yielded Bison priscus DNA, whereas Western Portel cave coprolites contained cave ... : NGS Amplicons reads of: Bison Priscus (Enlène cave) Bison schoetensacki (Le Portel cave), Bison schoetensacki (Roc-en-Pail), Rangifer tarandus (Roc-en-Pail) ...