Data from: Real-time assessment of hybridization between wolves and dogs: combining non-invasive samples with ancestry informative markers ...

• Main Authors: Godinho, Raquel, López-Bao, José Vicente, Castro, Diana, Llaneza, Luís, Lopes, Susana, Silva, Pedro, Ferrand, Nuno
• Format: Dataset
• Language: English
• Published: Dryad 2014
• Subjects: Canis lupus familiaris; wolf; simulated genotypes; Canis lupus
• Online Access: https://dx.doi.org/10.5061/dryad.84ph3; https://datadryad.org/stash/dataset/doi:10.5061/dryad.84ph3

Wolves and dogs provide a paradigmatic example of the ecological and conservation implications of hybridization events between wild and domesticated forms. However, our understanding of such implications has been traditionally hampered by both high genetic similarity and the difficulties in obtaining tissue samples (TS), which limit our ability to assess ongoing hybridization events. To assess the occurrence and extension of hybridization in a pack of wolf-dog hybrids in Northwestern Iberia, we compared the power of 52 nuclear markers implemented on TS with a subset of 13 ancestry informative markers (AIMs) typed in non-invasive samples (NIS). We demonstrate that the 13 AIMs are as accurate as the 52 markers that were chosen without regard to the power to differentiate between wolves and dogs, also having the advantage of being rapidly screened on NIS. The efficiency of AIMs significantly outperformed ten random sets of similar size and an additional commercial set of 18 markers. Bayesian clustering analysis ... : Genotypes for Iberian Wolves and DogsThis file contains Iberian wolves, dogs and Barbanza individuals’ genotypes. Sheet “52 loci” contains genotypes for the whole set of markers on our dataset. Sheet “13 loci” contains genotypes for the 13 AIMs selected as the most suitable for studying wolfxdog hybridization in this project.Simulated genotypes for wolves and dogsThis file contains 13 sets of loci for 1000 simulated genotypes of wolves and dogs. Sheet “52 loci” contains simulated genotypes for the whole set of markers on our dataset. Sheet “13 AIMs” contains simulated genotypes for the 13 AIMs selected as the most suitable for studying hybridization in this project. Sheet “Set Finnzymes” contains simulated genotypes for the 18 markers available on a commercial kit for dog microsatellites genotyping. Sets 1 to 10 are subsets of 13 random selected markers across the whole dataset of 52 loci. ...