Data from: Water, water everywhere: environmental DNA can unlock population structure in elusive marine species ...

• Main Authors: Parsons, Kim M., Everett, Meredith, Dahlheim, Marilyn, Park, Linda
• Format: Dataset
• Language: English
• Published: Dryad 2018
• Subjects: Next Generation Sequencing; porpoise; Phocoena phocoena; Stock; cetacean; Alaska
• Online Access: https://dx.doi.org/10.5061/dryad.77h8fd8; https://datadryad.org/stash/dataset/doi:10.5061/dryad.77h8fd8

Determining management units for natural populations is critical for effective conservation and management. However, collecting the requisite tissue samples for population genetic analyses remains the primary limiting factor for a number of marine species. The harbor porpoise (Phocoena phocoena), one of the smallest cetaceans in the Northern Hemisphere, is a primary example. These elusive, highly mobile small animals confound traditional approaches of collecting tissue samples for genetic analyses, yet their nearshore habitat makes them highly vulnerable to fisheries bycatch and the effects of habitat degradation. By exploiting the naturally shed cellular material in seawater and the power of next generation sequencing, we develop a novel approach for generating population-specific mitochondrial sequence data from environmental DNA (eDNA) using surface seawater samples. Indications of significant genetic differentiation within a currently recognized management stock highlights the need for dedicated eDNA ... : Phocoena_CR_sequences_Dryad_040418Control region sequences resolved from Alaska harbor porpoise. ...