Heat stress responses of the kelp Laminaria digitata (Phaeophyceae) across Northeast Atlantic populations: growth, biochemistry, chlorophyll fluorescence, pigments ...

• Main Authors: Liesner, Daniel, Fouqueau, Louise, Valero, Myriam, Roleda, Michael Y., Pearson, Gareth A., Bischof, Kai, Valentin, Klaus, Bartsch, Inka
• Format: Dataset
• Language: English
• Published: Dryad 2020
• Subjects: Laminaria digitata; Carbon; Pigments; xanthophyll; Chlorophyll; Fluorescence; Arctic; Bodø; Helgoland; Norway; Tromsø; Kongsfjord*; Kongsfjorden; Northeast Atlantic; Spitsbergen
• Online Access: https://dx.doi.org/10.5061/dryad.73n5tb2ts; https://datadryad.org/stash/dataset/doi:10.5061/dryad.73n5tb2ts

To understand the thermal plasticity of a coastal foundation species across its latitudinal distribution, we assess physiological responses to high temperature stress in the kelp Laminaria digitata in combination with population genetic characteristics and relate heat resilience to genetic features and phylogeography. We hypothesize that populations from Arctic and cold-temperate locations are less heat resilient than populations from warm distributional edges. Using meristems of natural L. digitata populations from six locations ranging between Kongsfjorden, Spitsbergen (79°N), and Quiberon, France (47°N), we performed a common-garden heat stress experiment applying 15°C to 23°C over eight days. We assessed growth, photosynthetic quantum yield, carbon and nitrogen storage, and xanthophyll pigment contents as response traits. Population connectivity and genetic diversity were analysed with microsatellite markers. Results from the heat stress experiment suggest that the upper temperature limit of L. digitata ... : We collected 30–35 fertile L. digitata sporophytes from the low intertidal zone, ensuring a distance of >1 m between samples (for the samples collected by diving in Spitsbergen, this was not guaranteed), in each of the following locations during summer: Stuphallet, Kongsfjorden, Spitsbergen, Norway (SPT, 78.975 N, 11.633 E; 16 July 2019; approximate SST at time of sampling: 6.5°C); north of Tromsø, Norway (TRO, 69.790 N, 19.054 E; 14 August 2018; 8.5°C); Bodø, Norway (67.284 N, 14.383 E; 12 June 2018; 9°C); Helgoland, Germany (HLG, 54.178 N, 7.893 E; 13 August 2018; 18°C); Roscoff, France (ROS, 48.727 N, 4.005 W; 11 September 2018; 16.5°C) and Quiberon, France (QUI, 47.470 N, 3.091 W; 10 September 2018; 16°C). ...