Data from: Fine-scale temporal analysis of genotype-dependent mortality at settlement in the Pacific oyster Crassostrea gigas ...

• Main Author: Plough, Louis V.
• Format: Dataset
• Language: English
• Published: Dryad 2019
• Subjects: larval; Metamorphosis; Crassostrea gigas; Pacific oyster
• Online Access: https://dx.doi.org/10.5061/dryad.6fm12; https://datadryad.org/stash/dataset/doi:10.5061/dryad.6fm12

Settlement and metamorphosis mark a critical transition in the life cycle of marine invertebrates, during which substantial mortality occurs in both field and laboratory settings. Previous pair-crossing experiments with the Pacific oyster Crassostrea gigas have revealed significant selective or genotype-dependent mortality around the metamorphic transition, but the fine-scale nature and timing of this mortality is not known, particularly whether it occurs before, during or after metamorphosis. In this laboratory study, microsatellite marker segregation ratios were followed daily throughout the settlement and metamorphosis of an F2 cross of the Pacific oyster to examine the fine-scale patterns of genotype dependent mortality at this transition and whether settlement timing (early vs. late) might be under genetic control and affect inference of genotype dependent mortality. Settlement occurred over nine days (day 18 to day 27 post-fertilization) with 68% of individuals settling either early (day 19) or late ... : Genotype Data for oyster spat (C. gigas) from early and late settlementThis excel sheet contains microsatellite marker genotypes for oyster spat settling on day 19 or day 24. Genotypes were called by eye (and compared to parent genotypes) on a ABI 377 prism instrument using the Genescan software. These data form the basis of the QTL mapping analyses and chi-square comparisons of early vs. late settlement in Plough 2018. Column A contains the individual Spat ID, and columns C through BK contain the genotype data. Microsatellite marker names are prefixed by "Cg" and are presented in pairs of columns for each marker: one for spat settling on day 19, the other for day 24 (Row 1). Row 2 contains the cross-type of the parents (e.g. abxab) and Row 3 contains the C. gigas linkage group (LG) information for a given marker (LGs 1-10).Plough JEMBE Spat genotype data DRYAD.xlsx ...