Asimina triloba genetic data ...

• Main Authors: Trapnell, Dorset, Wyatt, Graham, Hamrick, Jim
• Format: Dataset
• Language: English
• Published: Dryad 2021
• Subjects: Asimina triloba; clonality; pawpaw; pre-Columbian peoples; fine-scale spatial genetic structure; Aldrich; Doyle; Ice Sheet
• Online Access: https://dx.doi.org/10.5061/dryad.5x69p8d3g; https://datadryad.org/stash/dataset/doi:10.5061/dryad.5x69p8d3g

Dispersal and colonization are among the most important ecological processes for species persistence as they allow species to track changing environmental conditions. During the last glacial maximum (LGM), many cold-intolerant Northern Hemisphere plants retreated to southern glacial refugia. During subsequent warming periods, these species expanded their ranges northward. Interestingly, some tree species with limited seed dispersal migrated considerable distances after the LGM ~19,000 year before present (YBP). It has been hypothesized that indigenous peoples may have dispersed valued species, in some cases beyond the southern limits of the Laurentide Ice Sheet. To investigate this question we employed a molecular genetics approach on a widespread North American understory tree species whose fruit was valued by indigenous peoples. Twenty putative anthropogenic (near pre-Columbian habitations) and 62 wild populations of Asimina triloba (pawpaw), which produces the largest edible fruit of any North American ... : Genomic DNA was extracted from frozen leaf tissue (~ 0.05 g) using a modified CTAB protocol (Doyle & Doyle, 1983) and DNA quality and quantity were evaluated using an ND-1000 Nanodrop® spectrophotometer. Nine nuclear microsatellite loci (Lu et al., 2011; Table S2) were PCR amplified in 12.5 µL reaction volumes containing 3.25 µL molecular grade ddH2O, 2.5 µL 5X One Taq® standard reaction buffer (New England Biolabs, Ipswich, MA), 0.35 µL 25 mM MgCl solution (Sigma-Aldrich), 1.25 µL 10X (1 mg/mL) bovine serum albumin (Thermo-Fisher), 1 µL 2.5 mM dNTP mix (New England Biolabs, Ipswich, MA), 1 µL primer mix (0.5 µM CAG-labeled primer and 5 µM unlabeled primer), 0.45 µL 10 µM universal dye-labeled primer, 0.2 µL One Taq® Hot Start DNA polymerase (New England Biolabs, Ipswich, MA), and 2.5 µL of diluted template DNA (20 ng/µL). A 3-primer PCR protocol was used whereby a CAG-tag sequence (Hauswaldt & Glenn, 2003) was added to the 5’ end of one primer and a third fluorescently labeled (FAM, HEX, or NED) ...