Data from: Smooth brome invasion increases rare soil bacterial species prevalence and alters soil bacterial community composition

• Main Authors: Piper, Candace L., Siciliano, Steven D., Winsley, Tristrom, Lamb, Eric G.
• Format: Dataset
• Language: English
• Published: Dryad 2015
• Subjects: Chthonomonadetes; Candidatus Phytoplasma; Flexibacteraceae; Nordella; Cyclobacteriaceae; Methylibium petroleiphilum; Sulfobacillaceae; Arthrospira fusiformis; Xanthomonadaceae; FAC88; Thioalkalivibrio; Amycolatopsis australiensis; Armatimonadales; Achillea millefolium; Streptomyces scabrisporus; Micrococcaceae; Artemisia frigida; Chlorobi; Spartobacteriales; Cryptosporangium japonicum; Microscilla marina; Carex filifolia; Rosa arkansana; Haliangiaceae; Rubrobacter taiwanensis; Coxiella; Azospirillum; Prosthecobacter vanneervenii; Chelatococcus; Methylobacterium sp.4-46; Waddlia; Aeromicrobium marinum; Fervidobacterium nodosum; Pulsatilla patens; Dehalococcoidetes; Bromus anomalus; Okibacterium fritillariae; Olsenella; Cirsium flodmanii; Dichelobacter; Sejongia; wb1_P06; Clostridiales; Rhodospirillaceae; Stigmatella; Flavobacteria; Sediment-1; Psychrobacter; Vicia americana var. minor; GAL15; Veillonellaceae; C6; Candidatus Solibacter; Chthoniobacter flavus; Xanthomonadales; Williamsiaceae; Pseudomonadaceae; Rhodobacterales; Methyloversatilis; Rubellimicrobium thermophilum; Desulfobacula; Tatlockia; Geobacter sp.M21; Phycisphaeraceae; Nitrospira sp.; Bdellovibrio; Kineosporiaceae; Geodermatophilus; Rhodoplanes; Dyella; Anaerospora hongkongensis; Actinoplanes; Mycobacteriaceae; Dyellagin sengisoli; Chlorobiales; Chondromyces robustus; Oscillochloris; Hydrogenophilus; Rubellimicrobium; Cryptosporangiaceae; Thermotogae; Chthonomonadales; Microthrixaceae; Streptosporangium longisporum; Brevibacillus; Candidatus Entotheonella; Chondromyces; Methylopila; Blastopirellula marina; Koeleria macrantha; Pascopyrum smithii; Bacillus; Bromus inermis; Dokdonia donghaensis; Enterobacteriales; OPB56; Lactobacillales; Streptophyta; SM1B09; Sva0725; Kitasatospora; Williamsia muralis; Gemmatimonadetes; Magnetococcus; Methylacidiphilales; MJK10; Couchioplanes; Desulfuromonas; SCA1145; Clostridiaceae; Arhodomonas; Bryantella formatexigens; Cellulosimicrobium; Chloroherpeton; Tragopogon dubius; Streptomyces cyaneofuscatus; Mycobacterium chlorophenolicum; Descurainia sophia; Nostocaceae; Neisseriaceae; Flavobacteriales; Facklamia; Legionella; Chloroflexaceae; SCA1170; Helcococcus ovis; Sulfurihydrogenibium; Rhodospirillales; Agrobacterium; Exiguobacterium sibiricum; WS3; Gemmata; Kutzneria; Thermopsis rhombifolia; Geobacillus; Porphyrobacter; WS6; Erythrobacteraceae; Chloroflexus sp.Y-400-fl; Methylocystaceae; CFB-26; Streptosporangium; Dechloromonas; Plantibacter; Hydrogenophilales; Kineococcus radiotolerans; Desulfovibrio senezii; Chromatiales; Blastomonas; Deferribacteraceae; Laceyella sacchari; Coriobacteriales; Armatimonadia; Mycoplana; Acinetobacter rhizosphaerae; Massilia timonae; Roseococcus; Sphingomonadaceae; Dactylosporangium; Actinopolysporaceae; Providencia heimbachae; Aquimarina muelleri; Sphingobacteria; Acetobacteraceae; Salix wolfii; Burkholderiales; Anaerolinea; GN02; Pseudomonas oleovorans; GN08; Emticicia; GN07; Cupriavidus gilardii; Pedomicrobium; Rhodovibrio; Williamsia; Nitrospiraceae; Nitrospirales; SM1D11; Iamiaceae; CCM11b; BPC110; Aquimarina; Desulfomicrobiaceae; Mycobacterium stomatepiae; Candidatus Methylacidiphilum; Thiocystis; Candidatus Solibacterusitatus; Nocardia exalbida; Arthrospira; Gemmatimonadales; Actinoplanes globisporus; Halothermothrix orenii; Exiguobacterium; Bljii12; Anaerospora; Marinomonas; Xanthomonas; Gemmatales; Methylobacteriaceae; Alteromonadaceae; Cupriavidus pinatubonensis; Nautiliaceae; Thermodesulfovibrionaceae; Geobacter metallireducens; Bacilli; OPB80; Labrys; Antennaria parvifolia; Nautiliales; Methylobacterium; Cardiobacteriaceae; Dethiosulfovibrionaceae; Rhizobiales; Desulfuromonadaceae; Labrenzia; Flavobacterium succinicans; Solibacterales; Luteibacter rhizovicinus; Pirellula; Gemmatimonadaceae; Sphingosinicella; Chitinophaga; Microcoleus; Desulfovibrio magneticus; Thermoplasmatales; GN12; Flexibacter flexilis; Salinispora; Bradyrhizobium canariense; Sporolactobacillus kofuensis; Saprospiraceae; Euglenozoa; Rhodovibrio salinarum; Exiguobacterales; Actinoplanes durhamensis; Oxalobacteraceae; Bartonellaceae; Methylibium; CH21; Rhodospirillum; Rhodocyclaceae; Erythrobacter aquimaris; Rhodoferax; LD19; Thermosipho; Plesiocystis; Pantoea citrea; Rhodobiaceae; Helcococcus; Elusimicrobia; Deinococcus papagonensis; Planktothricoides raciborskii; Armatimonadetes; Kaistia; Virgisporangium ochraceum; AKIW874; Virgisporangium; Opitutales; Ignatzschineria; Luteolibacter; Gemmataceae; Chelativorans; Trichodesmium; Rickettsiales; Thermomonas; SR1; Dehalococcoidales; Methylococcales; Cellulomonadaceae; Caloramator; Zhouia amylolytica; Anaeromyxobacter; Lysobacter taiwanensis; Bacillus soli; Desulfomicrobium orale; Erysimum inconspicuum; Streptomyces ahygroscopicus; Yersinia; Leptonema illini; Frankiaceae; Deinococcus; Nannocystis exedens; Aurantimona saltamirensis; Cystobacter fuscus; Nassella viridula; Phormidiaceae; Thermosinus carboxydivorans; Halanaerobiales; ClostridialesFamilyXI.IncertaeSedis; Dyadobacter; Acidimicrobiales; Chlorobiaceae; Burkholderia sordidicola; Bartonella washoensis; Aeromicrobium; Fibrobacteres; Flammeovirgaceae; Methylacidiphilaceae; Deinococcales; Spartobacteriaceae; Mycoplasma; Modestobacter; Dehalococcoides sp.BAV1; Streptomyces tumescens; Paenibacillus sabinae; Providencia; Sphingosinicella microcystinivorans; Alteromonadales; Rubrobacter xylanophilus; Actinoallomurus; Streptomyces cuspidosporus; Rubrobacteraceae; TIBE07; Beijerinckiaceae; Hydrogenophilus halorhabdus; Slackia exigua; ZB2; Anaerolinaceae; Methylobacterium organophilum; MC18; Enterococcus; Mycobacterium; Rhodopseudomonas; Simkaniaceae; Rhizobium; Stenoxybacter; SB-34; Herpetosiphonaceae; Actinobaculum; Trabulsiella; Coxiellaceae; Blastococcus; Arabis divicarpa; Pirellula staleyi; Pseudonocardia; Massilia; Phaeospirillum; Rhodobacteraceae; Nostoc; Pseudomonas fragi; Rhodococcus; Loripes lacteus gill symbiont; Actinomadura nitritigenes; Bacillaceae; Actinomadura; Saccharomonospora; Zhouia; Brevundimonas; Acidobacteriales; Actinomycetales; Brevibacillus levickii; Anaerolineae; Chlamydiae; Desulfovibrio; Streptomyces reticuliscabiei; Taraxacum officinale; Azohydromonas; Anaerolineales; Hydrogenophilaceae; Rubrobacter radiotolerans; Halothermothrix; Candidatus Nitrososphaera; Actinomadura madurae; Marinomonas aquimarina; Clostridium ultunense; wb1_H11; Waddliaceae; Methylobacterium podarium; Verrucomicrobiales; Fibrobacter intestinalis; OP3; Verrucosispora gifhornensis; Actinokineospora diospyrosa; Pimelobacter; Balneimonas; Okibacterium; Cytophaga; Sphingobium yanoikuyae; MVP-88; Actinoplanes ferrugineus; Rhodocyclales; Natranaerobiaceae; Candidatus Nitrososphaeragargensis; Aurantimonas; Streptomyces stelliscabiei; Pasteurella aerogenes; Opitutus; soil bacterial community; Carex stenophylla; Rhodanobacter lindaniclasticus; Cohnella; Microscilla; Pseudoalteromonas atlantica; Steroidobacter; Spartobacteria; Cerastium arvense; Oceanospirillales; Hesperostipa curtiseta; RB384; Mobiluncus mulieris; Actinoplanes italicus; Rubrobacter; Lentisphaerae; Epsilonproteobacteria; Salinibacillus; Tatlockia maceachernii; Pasteurellaceae; Porphyrobacter meromictius; Bacillus nealsonii; Deferribacteres; Jonquetella; MC47; mle1-48; Thermomicrobia; Cryptosporangium; Legionella pneumophila; Salinispora arenicola; Planifilum fulgidum; Laribacter hongkongensis; Dictyoglomus turgidum; Synergistia; Campylobacter; S1a-1H; Oscillochloris trichoides; Streptomyces yanglinensis; euryarchaeota; Ignatzschineria larvae; Ralstonia solanacearum; Deferribacterales; Solibacteraceae; Enterobacterialendo symbiont of Drosicha corpulenta; Methylophilales; Thermobaculum terrenum; Streptosporangium violaceochromogenes; Nannocystis; Idiomarina; Nitrosomonadales; Ecwsrb050; Echinicola pacifica; Leptospira; Dichelobacter nodosus; Thiomicrospira crunogena; Kosmotoga; Singulisphaera acidiphila; Stramenopiles; Caulobacteraceae; Holophagales; Enterococcus dispar; Bosea; Terriglobus; Aquicella; Brachyspirae; Leptospira interrogans; Thermodesulfovibrio yellowstonii; mle1-12; Mesorhizobium; Dokdonella; Agromyces; Streptomyces nitrosporeus; Pirellulaceae; Planctomycetes; Glaciecola; Thermoanaerobacterales; Actinomycetospora; Burkholderiaceae; Prosthecobacter debontii; Afipiagenosp.9; Myxococcaceae; Pseudonocardia yunnanensis; Rhodococcus globerulus; Deinococcaceae; Thermomicrobiales; Devosia; Lysobacter; Campylobacter showae; FFCH6980; Sporosarcina; Candidatus Haliscomenobactercalcifugiens; WPS-2; PRR-11; PRR-10; Luteibacter; Thermotoga lettingae; Verrucomicrobia; PRR-12; FFCH4570; Leptospirae; Chloroflexi; Acidimicrobiaceae; Ectothiorhodospira sp.BogoriaRed; Burkholderia; Dokdonella koreensis; Ectothiorhodospiraceae; Aeromicrobium fastidiosum; NKB19; Polaromonas; Geobacter; Rhodospirillum centenum; Leptospirales; Kueneniae; GKS2-174; Marinospirillum; Cellulomon asxylanilytica; Euzebia; Protochlamydia naegleriophila; Acidobacteria-5; Cupriavidus; Aminobacter; Enterobacter radicincitans; Sphingobacteriaceae; Campylobacterales; Nautilia profundicola; Burkholderia glathei; Promicromonosporaceae; Methanosarcina; Streptomyces radiopugnans; SJA-36; Halobacteriales; Labrenzia aggregata; Aerococcaceae; Frankia sp.EAN1pec; Streptomyces flavotricini; Sphingobium; Alphaproteobacteria; Xanthobacteraceae; Chromatiaceae; Nitrososphaeraceae; Roseiflexales; Thermosinus; Myxococcales; Erigeron glabellus; Sphingobacteriales; Kouleothrix; Thermotogaceae; Oceanobacillus; Chlorobia; Bradyrhizobium; Halostagnicola; DS-18; Sulfurihydrogenibium azorense; Poa palustris; PW285; Synechococcus; Galium boreale; Sphingobium herbicidovorans; Bouteloua gracilis; Azospirillum sp.B510; Nitrosomonadaceae; Picrophilus; Methanosarcina barkeri; Nocardia fluminea; EB1017; Kaistobacter; Sphingomonas azotifigens; Flavisolibacter; Psychrobacter sp.PRwf-1; Desulfovibrionales; Festuca altaica ssp. Hallii; koll11; Nitrospirae; koll13; Microbacteriaceae; Halobacteria; Solirubrobacter; Herpetosiphon; Bacillus longiquaesitum; NB1-i; NB1-j; Levilinea; Paenibacillus terrae; Bacillus coahuilensis; envOPS12; SJA-28; Roseiflexus; Planotetraspora; Rickettsiella; crenarchaeota; Holophagae; Nannocystaceae; Dolo_23; Comamonadaceae; WD294; Novosphingobium; Thermodesulfovibrio; OM190; Gemmata obscuriglobus; Lactobacillaceae; Skermanella; Mobiluncus curtisii; Anaeromyxobacter sp.Fw109-5; Sporolactobacillus nakayamae; Thioclava; Rickettsiella grylli; Kineococcus; Hydrogenophilus thermoluteolus; Thermotoga; Jonquetella anthropi; Piscirickettsiaceae; Ideonella; Candidatus Phytoplasmamali; Cellvibrio japonicus; Muhlenbergia richardsonis; Thiomicrospira; Azospirillum melinis; Nautilia; CTD005-82B-02; Bogoriellaceae; Pseudomonas umsongensis; Desulfurellales; Chloracidobacteria; Methanosarcinaceae; Armatimonas; Flexibacter; Mollicutes; Methylobacterium radiotolerans; Brachyspira pilosicoli; RB25; Coriobacteriaceae; Microbispora rosea; Bellilinea; Acidovorax; Dictyoglomus; Thermoactinomycetaceae; Deinococcus deserti; Streptosporangiaceae; Astragalus flexuosus; Cardiobacteriales; Amycolatopsis; Legionellales; Frateuria; Magnetococcus sp.MC-1; AD3; HN1-15; Picrophilaceae; BRC1; Pedobacter; Sulfurihydrogenibium sp.YO3AOP1; Promicromonospora; Planctomycetales; Juncus balticus; Desulfurellaceae; Desulfovibrionaceae; Flavobacterium; Roseivirga; Blastococcus aggregatus; Nocardioides oleivorans; iii1-8; Leptospiraceae; Campanula rotundifolia; Luteimonas; Sphingopyxis; Nocardioidaceae; Erythrobacter; Streptomyces spiroverticillatus; H39; Thermopallium; Idiomarinaceae; Desulfuromonadales; Thiotrichales; Acidobacteria; B07_WMSP1; Oceanospirillaceae; Elymus lanceolatus ssp. lanceolatus; Planctomycea; Pelobacter propionicus; Methanosarcinales; Entotheonellales; Solibacteres; Pasteurellales; Sphingomonadales; Mechercharimyces mesophilus; Sphingomonas wittichii; Alcanivoracaceae; Agoseris glauca; Sporolactobacillaceae; Geobacter psychrophilus; TM6; Oscillatoriales; Caminibacter; TM7; Knoellia subterranea; Pseudomonas mandelii; Rhodomicrobium; Facklamia sourekii; Halobacillus virgiliensis; Rhodanobacter; Symphyotrichum ericoides; Carex pensylvanica; Slackia; Bdellovibrio bacteriovorus; Lactobacillus kefiranofaciens; Labrys methylaminiphilus; Jiangella; Deltaproteobacteria; Acholeplasmataceae; Sporolactobacillus; SC72; Verrucomicrobia subdivision 3; Frankia; Tenericutes; Thermotogales; Bacillales; Lachnospiraceae; Intrasporangiaceae; Candidatus Protochlamydia; MIZ46; Methylococcus; Polyangiaceae; Laceyella; Actinomycetaceae; Aurantimonadaceae; Saccharothrix; Ectothiorhodospira; Caminibacter mediatlanticus; Synergistetes; Fibrobacterales; Paenibacillus chondroitinus; Roseisalinus; SM2F11; Roseiflexus castenholzii; Caldilineaceae; Kineosporia; Thermopallium natronophilum; Halobacillus; Thermobaculum; Rubrobacterales; Chthonomonas calidirosea; Bacteroidales; Opitutaceae; Micromonospora; Nitratiruptor sp.SB155-2; Kouleothrixaceae; Armatimonadaceae; Oscillatoriophycideae; Neisseriales; Sporichthyaceae; LD1-PA13; Azomonas macrocytogenes; Planktothricoides; Natranaerobiales; Mycoplasma phocicerebrale; Dictyoglomaceae; Opitutaceae bacterium TAV2; Flavobacteriaceae; structural equation modeling; Ralstonia; Peptococcaceae; Thermomonosporaceae; Pilimelia; Sporichthya; Mycoplasmataceae; FFCH393; Bradyrhizobiaceae; JG37-AG-70; Helictotrichon hookerii; Dehalobacteriaceae; Phyllobacteriaceae; Dehalococcoidaceae; Acidimicrobiumf errooxidans; Moraxellaceae; Caldilinea; Burkholderia oklahomensis; Aquimonas; Sulfobacillus; Syntrophobacterales; Bacillus flexus; Methyloversatilis universalis; Thermobacula; Brachyspiraceae; Rhizobium multihospitium; Syntrophobacter; Brachyspira; Knoellia; Nocardia; Idiomarina loihiensis; Koribacteraceae; Arabis hirsuta; Micromonosporaceae; Hesperostipa comata; Carex obtustata; Solimonas; Lamprocystis; Methylacidiphilae; Rhizobiaceae; Niabella; Alicyclobacillaceae; Mesorhizobium huakuii; Thermobaculaceae; Microbispora; Amaricoccus; Bdellovibrionaceae; Bacteroidetes; Amycolatopsis mediterranei; Deinococci; Rhodopirellula; Oscillochloridaceae; Nonomuraea; Tatumella; Dictyoglomia; Actinobacteria; GCA004; Artemisia ludoviciana; S085; Streptomycetaceae; Dehalococcoides; Sphingomonas; Paenisporosarcina; Mechercharimyces; S15B-MN24; Elusimicrobiales; Pseudoxanthomonas; Thermoanaerobacteraceae; Lutibacterium; Nostocales; Isosphaeraceae; Streptomyces thermovulgaris; Catellato sporacitrea; Thiocystis gelatinosa; Proteobacteria; Sphingomonas melonis; Olsenella profusa; Penstemon procerus; AKYG885; Oribacterium sp.oraltaxon078; Methylobacterium adhaesivum; Syntrophobacteraceae; Androsace sepentrionalis; Desulfovibrio bastinii; Planctomycetaceae; S47; Desulfofaba; Chlamydiales; Laribacter; Alcaligenaceae; Micromonospora echinaurantiaca; Thermaerobacter subterraneus; Archangium; Leptonema; Dictyoglomi; Micromonospora aurantiaca; Planifilum; Chthonomonas; Phycisphaerales; Bacteroidia; Cellulomonas; Aquificae; Pelotomaculum; Geodermatophilaceae; Iamia; Thermoplasmata; Clostridium; Stellaria longifolia; Symphyotrichum laeve; Paenibacillus sp.JDR-2; Methanomicrobia; PAUC37f; Nocardiaceae; Synergistales; Roseisalinus antarcticus; Myxococcus stipitatus; Herpetosiphon aurantiacus; Nitrospira; Pseudomonas cuatrocienegasensis; Pirellulales; Chroococcales; Fervidobacterium; Cupriavidus oxalaticus; Aquamonas; Chloroplast; Sinobacteraceae; Fibrobacteraceae; Legionellaceae; Geobacteraceae; Mesorhizobium albiziae; Verrucosispora; Pseudonocardiaceae; Gemmatimonas; Pelotomaculum thermopropionicum; Amycolatopsis lactamdurans; GOUTA4; Pseudomonas boreopolis; Bdellovibrionales; Arthrobacter; Thermaerobacter; Pseudomonadales; agg27; Caulobacterales; Thermobaculales; Desulfomicrobium; Thermosipho melanesiensis; Linum lewisii; Actinoplanes teichomyceticus; Actinoallomurus iriomotensis; Rosa woodsii; Mycobacterium gordonae; Mycoplasmatales; Roseomonas; Saccharomonospora viridis; Edaphobacter; Cystobacter; Hydrogenothermaceae; Anaerobaculaceae; PBS-25; Phycisphaerae; Frateuria aurantia; Solirubrobacterales; Verrucomicrobiaceae; Sphingopyxis macrogoltabida; Anemone canadensis; Bacteroides; Georgenia; Candidatus Cardiniumhertigii; Patulibacteraceae; Marmoricola; Bacillus weihenstephanensis; Aphanizomenon; Chlorophyta; Candidatus Xiphinematobacter; Mycoplasma conjunctivae; Spirochaetes; Desulfobacteraceae; CL500-29; Chloroflexus; Spirosoma; Inquilinus; Chthonomonadaceae; Sphaerlacea coccinea; Firmicutes; CL500-15; Actinopolymorpha; Phycicoccus; Natranaerobius thermophilus; Oxalobacter; Thermosipho africanus; Nostocophycideae; Paenibacillus borealis; Thermi; Hyphomicrobium; evenness; Symbiobacteriaceae; Actinosynnemataceae; Enterococcaceae; Methylococcus capsulatus; Thermoanaerobacter; Oribacterium; Phyllobacterium; S0208; TK17; Euzebiales; Herpetosiphonales; Cellvibrio; Chloroherpeton thalassium; SOGA31; Hyphomicrobiaceae; Nakamurellaceae; invasive ecology; Opitutae; Aquificales; Alicyclobacillus; vadinHA49; Cyanobacteria; MVS-40; Haliscomenobacter; Methylobacterium fujisawaense; Synechococcus sp.PCC7002; Syntrophobacter fumaroxidans; Phenylobacterium; Caulobacter; Lactobacillus; Streptomyces antibioticus; WCHB1-50; Chloroflexi-4; Cryobacterium; Prosthecobacter; Astragalus agrestis; Dictyoglomales; Chloroflexales; Desulfuromonas svalbardensis; Anaerobaculum; Marinospirillum insulare; Acidobacteriaceae; Solirubrobacteraceae; Sporosarcina aquimarina; Entotheonellaceae; Acholeplasmatales; RA13C7; Cystobacteraceae; Verrucomicrobiae; Natranaerobius; SBRH58; Sonchus arvensis; SJA-4; Chthoniobacter; Singulisphaera; Edaphobacter modestum; Azomonas; Fibrobacter; Kribbella; Planctomyces; Antarc*; antarcticus
• Online Access: https://dx.doi.org/10.5061/dryad.5sn8m; http://datadryad.org/stash/dataset/doi:10.5061/dryad.5sn8m

Plant and soil communities are tightly linked, but the mechanisms by which the invasion of an exotic plant and the resulting shifts in plant diversity and productivity influence soil bacterial community structure remain poorly understood. We investigated the effects of invasive smooth brome (Bromus inermis) on grassland soil bacterial community structure using massively-parallel sequencing of the 16S rRNA gene to determine bacterial community richness, evenness, composition, and beta diversity (UniFrac indices) of soils collected along a gradient of smooth brome abundance. We evaluated several hypotheses including: a) that the declines in native plant diversity associated with smooth brome invasion would cause declines in bacterial community diversity, and b) that mechanisms driving smooth brome effects on bacterial community structure involved altered soil edaphic properties rather than preferential invasion in areas of high soil nitrogen and distinct soil microbial communities. Smooth brome invasion led to increased soil nitrogen, soil carbon and root biomass. Bacterial evenness and bacterial richness increased with increasing smooth brome cover, while bacterial beta diversity declined. We found no evidence of a dominant direct link between the alteration of soil edaphic properties by brome and the changes in the soil bacterial community. Rather, the main controls on the soil bacterial community were direct effects of pH and smooth brome that could not be linked to the edaphic changes. The most important effect of brome on the bacterial community was the selective suppression of dominant bacterial species, which allowed rarer bacteria to increase in relative abundance. Synthesis: Here we show that plant community composition influences bacterial community structure at a very fine scale, but that these changes are not due to altered soil total nitrogen or carbon content. The dominant direct effect of smooth brome invasion on soil communities suggests non-edaphic, i.e. inter and intra-trophic, interactions among smooth brome and non-bacterial components of the soil ecosystem are key drivers of soil community structure. Some of the data in this repository were also reported in the following paper: Piper, C.L., Lamb, E.G. & Siciliano, S.D. (In Press) Smooth brome changes gross soil nitrogen cycling processes during invasion of a rough fescue grassland. Plant Ecology. doi:10.1007/s11258-014-0431-y : Piper_plotdataFile Piper_plotdata This file includes all aboveground plant community measurements.Piper_soildataFile Piper_soilsdata This file includes all soil variables measured in both the A and B soil horizons at each plot.Piper_plantcommunityFile Piper_plantcommunity This file includes estimates of percent cover for all vascular plant species encountered in the plots.Piper_Ahorizon_bacteria_phylumFile Piper_Ahorizon_bacteria_phylum This file includes abundance estimates for bacterial OTUs in A-horizon soil samples clustered at the phylum level. Taxonomic assignment according to the Greengenes 2011 taxonomyPiper_Ahorizon_bacteria_orderFile Piper_Ahorizon_bacteria_order This file includes abundance estimates for bacterial OTUs in A-horizon soil samples clustered at the order level. Taxonomic assignment according to the Greengenes 2011 taxonomyPiper_Bhorizon_bacteria_phylumFile Piper_Bhorizon_bacteria_phylum This file includes abundance estimates for bacterial OTUs in B-horizon soil samples clustered at the phylum level. Taxonomic assignment according to the Greengenes 2011 taxonomyPiper_Bhorizon_bacteria_phylym.csvPiper_Bhorizon_bacteria_orderFile Piper_Bhorizon_bacteria_order This file includes abundance estimates for bacterial OTUs in B-horizon soil samples clustered at the order level. Taxonomic assignment according to the Greengenes 2011 taxonomyPiper_BacterialOTU_taxonomyFile Piper_bacterialOTU_taxonomy This file summarizes the taxonomy of bacterial OTUs observed in this study. Taxonomic assignment of OTUs was achieved by BLAST comparison of the seed sequences against the Greengenes 2011 database (McDonald et al., 2012) by trimming the Greengenes sequences to the same region as the seed sequences to improve assignment (Werner et al., 2012). Numbers in brackets indicate the confidence in which a particular name was assigned e.g. (100) is 100% confidence that the correct name was assigned Some OTUs are unnamed at some level of classification (e.g. f__(100) indicates that an OTU belongs to a certain unnamed family). Alternatively, an OTU may be "unclassified" denoting that the species could not be fit with any known bacterial groups.Piper_BacterialOTU_by_sample_matrixFile Piper_bacterial_OTU_sample_matrix This file contains the raw OTU by sample matrix for all soil samples. OTU_names are explained in the file Piper_bacterial_OTU_taxonomy The samples were analysed as a subsampled dataset in which a total of 114 samples were analysed, standardised to 2550 reads each (singleton OTUs were removed from the dataset, therefore the abundance totals don't all add up to 2550) This is a matrix of each OTU at species level (0.03 dissimilarity) and its standardised (to 2550 reads) occurrence in each sample.