Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: the complex case of Carex bigelowii s.lat. in Norway ...
There is a growing demand for ecological restoration using suitable seeds following international standards or national legal demands for local seed-sourcing. However, before selecting the appropriate geographic origin of seeds, it is vital to explore taxonomic complexity related to the focal taxa....
Main Authors: | , , |
---|---|
Format: | Dataset |
Language: | English |
Published: |
Dryad
2021
|
Subjects: | |
Online Access: | https://dx.doi.org/10.5061/dryad.4b8gthtdr https://datadryad.org/stash/dataset/doi:10.5061/dryad.4b8gthtdr |
id |
ftdatacite:10.5061/dryad.4b8gthtdr |
---|---|
record_format |
openpolar |
spelling |
ftdatacite:10.5061/dryad.4b8gthtdr 2024-02-04T09:59:36+01:00 Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: the complex case of Carex bigelowii s.lat. in Norway ... Westergaard, Kristine Bakke Kyrkjeeide, Magni Olsen Brandrud, Marie Kristine 2021 https://dx.doi.org/10.5061/dryad.4b8gthtdr https://datadryad.org/stash/dataset/doi:10.5061/dryad.4b8gthtdr en eng Dryad https://dx.doi.org/10.5281/zenodo.5603319 Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 FOS Biological sciences double-digest restriction site-associated DNA sequencing ddRAD-seq Carex bigelowii Dataset dataset 2021 ftdatacite https://doi.org/10.5061/dryad.4b8gthtdr10.5281/zenodo.5603319 2024-01-05T04:39:59Z There is a growing demand for ecological restoration using suitable seeds following international standards or national legal demands for local seed-sourcing. However, before selecting the appropriate geographic origin of seeds, it is vital to explore taxonomic complexity related to the focal taxa. We used ddRAD-seq to screen genomic diversity within Carex bigelowii s.lat. focussing on Norway. This species complex is considered a candidate for seeding, but presents considerable morphological, ecological, and genetic variation. The genetic structure of 132 individuals of C. bigelowii s.lat., including C. nigra as an outgroup, was explored using ordinations, clustering analyses, and a genetic barrier algorithm. Two highly divergent clusters were evident, supporting the recognition of two taxonomic units ‘C. dacica’ and ‘subsp. bigelowii’. Previously defined seed-sourcing regions for C. bigelowii s.lat. did not consider the known taxonomic complexity, and therefore interpreted the overall genetic structure as ... : Leaf samples from 139 individuals of Carex bigelowii s.lat. collected from 33 sites across its Norwegian distribution and 16 sites outside Norway. Information on collection sites, lat and long, collectors and voucher IDs are all given in Table 1 (uploaded to DRYAD). Preparation of ddRAD-seq libraries, de novo assembly of loci, variant calling and filtering (including MAF sensitivity analyses) are described in the publication. Two datasets are uploaded: dataset_A_all_het0.8_r90_maf0.01.vcf includes all SNPs for ordinations (15,095 SNPs, including linked markers from the same locus) dataset_B_all_het0.8_r90_maf0.01_single_snp.stru includes only one SNP per locus (5,134 SNPs, approximating unlinked markers) ... Dataset Carex bigelowii DataCite Metadata Store (German National Library of Science and Technology) Norway |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
FOS Biological sciences double-digest restriction site-associated DNA sequencing ddRAD-seq Carex bigelowii |
spellingShingle |
FOS Biological sciences double-digest restriction site-associated DNA sequencing ddRAD-seq Carex bigelowii Westergaard, Kristine Bakke Kyrkjeeide, Magni Olsen Brandrud, Marie Kristine Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: the complex case of Carex bigelowii s.lat. in Norway ... |
topic_facet |
FOS Biological sciences double-digest restriction site-associated DNA sequencing ddRAD-seq Carex bigelowii |
description |
There is a growing demand for ecological restoration using suitable seeds following international standards or national legal demands for local seed-sourcing. However, before selecting the appropriate geographic origin of seeds, it is vital to explore taxonomic complexity related to the focal taxa. We used ddRAD-seq to screen genomic diversity within Carex bigelowii s.lat. focussing on Norway. This species complex is considered a candidate for seeding, but presents considerable morphological, ecological, and genetic variation. The genetic structure of 132 individuals of C. bigelowii s.lat., including C. nigra as an outgroup, was explored using ordinations, clustering analyses, and a genetic barrier algorithm. Two highly divergent clusters were evident, supporting the recognition of two taxonomic units ‘C. dacica’ and ‘subsp. bigelowii’. Previously defined seed-sourcing regions for C. bigelowii s.lat. did not consider the known taxonomic complexity, and therefore interpreted the overall genetic structure as ... : Leaf samples from 139 individuals of Carex bigelowii s.lat. collected from 33 sites across its Norwegian distribution and 16 sites outside Norway. Information on collection sites, lat and long, collectors and voucher IDs are all given in Table 1 (uploaded to DRYAD). Preparation of ddRAD-seq libraries, de novo assembly of loci, variant calling and filtering (including MAF sensitivity analyses) are described in the publication. Two datasets are uploaded: dataset_A_all_het0.8_r90_maf0.01.vcf includes all SNPs for ordinations (15,095 SNPs, including linked markers from the same locus) dataset_B_all_het0.8_r90_maf0.01_single_snp.stru includes only one SNP per locus (5,134 SNPs, approximating unlinked markers) ... |
format |
Dataset |
author |
Westergaard, Kristine Bakke Kyrkjeeide, Magni Olsen Brandrud, Marie Kristine |
author_facet |
Westergaard, Kristine Bakke Kyrkjeeide, Magni Olsen Brandrud, Marie Kristine |
author_sort |
Westergaard, Kristine Bakke |
title |
Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: the complex case of Carex bigelowii s.lat. in Norway ... |
title_short |
Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: the complex case of Carex bigelowii s.lat. in Norway ... |
title_full |
Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: the complex case of Carex bigelowii s.lat. in Norway ... |
title_fullStr |
Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: the complex case of Carex bigelowii s.lat. in Norway ... |
title_full_unstemmed |
Using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: the complex case of Carex bigelowii s.lat. in Norway ... |
title_sort |
using genomics to guide seed-sourcing at the right taxonomical level for ecological restoration projects: the complex case of carex bigelowii s.lat. in norway ... |
publisher |
Dryad |
publishDate |
2021 |
url |
https://dx.doi.org/10.5061/dryad.4b8gthtdr https://datadryad.org/stash/dataset/doi:10.5061/dryad.4b8gthtdr |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Carex bigelowii |
genre_facet |
Carex bigelowii |
op_relation |
https://dx.doi.org/10.5281/zenodo.5603319 |
op_rights |
Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 |
op_doi |
https://doi.org/10.5061/dryad.4b8gthtdr10.5281/zenodo.5603319 |
_version_ |
1789964494529626112 |