Data from: Carnivore diet analysis based on next-generation sequencing: application to the leopard cat (Prionailurus bengalensis) in Pakistan ...
Diet analysis is a prerequisite to fully understand the biology of a species and the functioning of ecosystems. For carnivores, traditional diet analyses mostly rely upon the morphological identification of undigested remains in the feces. Here, we developed a methodology for carnivore diet analyses...
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ftdatacite:10.5061/dryad.443t4m1q 2024-02-04T10:04:10+01:00 Data from: Carnivore diet analysis based on next-generation sequencing: application to the leopard cat (Prionailurus bengalensis) in Pakistan ... Shehzad, Wasim Riaz, Tiayyba Miquel, Christian Poillot, Carole Shah, Safdar Coissac, Eric Taberlet, Pierre Nawaz, Muhammad Ali Pompanon, François 2011 https://dx.doi.org/10.5061/dryad.443t4m1q https://datadryad.org/stash/dataset/doi:10.5061/dryad.443t4m1q en eng Dryad https://dx.doi.org/10.1111/j.1365-294x.2011.05424.x Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 Mus musculus ribosomal DNA Columba livia Crocidura pullata Lepus capensis Hyperacrius wynnei Pucrasia macrolopha Corvus macrorhynchos Timaliidae Dendrocopos sp. Eoglaucomys fimbriatus Dryomys nitedula Prionailurus bengalensis Paa vicina Siluriformes species identification Lophura leucomelanos Alectoris Chukar Apodemus rusiges blocking primer Rattus rattus Gallus gallus Dataset dataset 2011 ftdatacite https://doi.org/10.5061/dryad.443t4m1q10.1111/j.1365-294x.2011.05424.x 2024-01-05T04:39:59Z Diet analysis is a prerequisite to fully understand the biology of a species and the functioning of ecosystems. For carnivores, traditional diet analyses mostly rely upon the morphological identification of undigested remains in the feces. Here, we developed a methodology for carnivore diet analyses based on next generation sequencing. We applied this approach to the analysis of the vertebrate component of leopard cat diet in two ecologically distinct regions in northern Pakistan. Despite being a relatively common species with a wide distribution in Asia, little is known about this elusive predator. We analyzed a total of 38 leopard cat feces. After a classical DNA extraction, the DNA extracts were amplified using primers for vertebrates targeting about 100 bp of the mitochondrial 12S rRNA gene, with and without a blocking oligonucleotide specific to the predator sequence. The amplification products were then sequenced on a next generation sequencer. We identified a total of 18 prey taxa, including eight ... : pb_bk_sequence_datapb_bk_filtered_dataFiltered data obtained with blocking oligonucleotide.pb_sequence_dataFile containing all unique sequences obtained with blocking oligonucleotide.pb_filtered_dataFiltered data obtained with blocking oligonucleotide. ... Dataset Rattus rattus DataCite Metadata Store (German National Library of Science and Technology) Chukar ENVELOPE(118.100,118.100,63.700,63.700) Paa ENVELOPE(-53.483,-53.483,66.017,66.017) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Mus musculus ribosomal DNA Columba livia Crocidura pullata Lepus capensis Hyperacrius wynnei Pucrasia macrolopha Corvus macrorhynchos Timaliidae Dendrocopos sp. Eoglaucomys fimbriatus Dryomys nitedula Prionailurus bengalensis Paa vicina Siluriformes species identification Lophura leucomelanos Alectoris Chukar Apodemus rusiges blocking primer Rattus rattus Gallus gallus |
spellingShingle |
Mus musculus ribosomal DNA Columba livia Crocidura pullata Lepus capensis Hyperacrius wynnei Pucrasia macrolopha Corvus macrorhynchos Timaliidae Dendrocopos sp. Eoglaucomys fimbriatus Dryomys nitedula Prionailurus bengalensis Paa vicina Siluriformes species identification Lophura leucomelanos Alectoris Chukar Apodemus rusiges blocking primer Rattus rattus Gallus gallus Shehzad, Wasim Riaz, Tiayyba Miquel, Christian Poillot, Carole Shah, Safdar Coissac, Eric Taberlet, Pierre Nawaz, Muhammad Ali Pompanon, François Data from: Carnivore diet analysis based on next-generation sequencing: application to the leopard cat (Prionailurus bengalensis) in Pakistan ... |
topic_facet |
Mus musculus ribosomal DNA Columba livia Crocidura pullata Lepus capensis Hyperacrius wynnei Pucrasia macrolopha Corvus macrorhynchos Timaliidae Dendrocopos sp. Eoglaucomys fimbriatus Dryomys nitedula Prionailurus bengalensis Paa vicina Siluriformes species identification Lophura leucomelanos Alectoris Chukar Apodemus rusiges blocking primer Rattus rattus Gallus gallus |
description |
Diet analysis is a prerequisite to fully understand the biology of a species and the functioning of ecosystems. For carnivores, traditional diet analyses mostly rely upon the morphological identification of undigested remains in the feces. Here, we developed a methodology for carnivore diet analyses based on next generation sequencing. We applied this approach to the analysis of the vertebrate component of leopard cat diet in two ecologically distinct regions in northern Pakistan. Despite being a relatively common species with a wide distribution in Asia, little is known about this elusive predator. We analyzed a total of 38 leopard cat feces. After a classical DNA extraction, the DNA extracts were amplified using primers for vertebrates targeting about 100 bp of the mitochondrial 12S rRNA gene, with and without a blocking oligonucleotide specific to the predator sequence. The amplification products were then sequenced on a next generation sequencer. We identified a total of 18 prey taxa, including eight ... : pb_bk_sequence_datapb_bk_filtered_dataFiltered data obtained with blocking oligonucleotide.pb_sequence_dataFile containing all unique sequences obtained with blocking oligonucleotide.pb_filtered_dataFiltered data obtained with blocking oligonucleotide. ... |
format |
Dataset |
author |
Shehzad, Wasim Riaz, Tiayyba Miquel, Christian Poillot, Carole Shah, Safdar Coissac, Eric Taberlet, Pierre Nawaz, Muhammad Ali Pompanon, François |
author_facet |
Shehzad, Wasim Riaz, Tiayyba Miquel, Christian Poillot, Carole Shah, Safdar Coissac, Eric Taberlet, Pierre Nawaz, Muhammad Ali Pompanon, François |
author_sort |
Shehzad, Wasim |
title |
Data from: Carnivore diet analysis based on next-generation sequencing: application to the leopard cat (Prionailurus bengalensis) in Pakistan ... |
title_short |
Data from: Carnivore diet analysis based on next-generation sequencing: application to the leopard cat (Prionailurus bengalensis) in Pakistan ... |
title_full |
Data from: Carnivore diet analysis based on next-generation sequencing: application to the leopard cat (Prionailurus bengalensis) in Pakistan ... |
title_fullStr |
Data from: Carnivore diet analysis based on next-generation sequencing: application to the leopard cat (Prionailurus bengalensis) in Pakistan ... |
title_full_unstemmed |
Data from: Carnivore diet analysis based on next-generation sequencing: application to the leopard cat (Prionailurus bengalensis) in Pakistan ... |
title_sort |
data from: carnivore diet analysis based on next-generation sequencing: application to the leopard cat (prionailurus bengalensis) in pakistan ... |
publisher |
Dryad |
publishDate |
2011 |
url |
https://dx.doi.org/10.5061/dryad.443t4m1q https://datadryad.org/stash/dataset/doi:10.5061/dryad.443t4m1q |
long_lat |
ENVELOPE(118.100,118.100,63.700,63.700) ENVELOPE(-53.483,-53.483,66.017,66.017) |
geographic |
Chukar Paa |
geographic_facet |
Chukar Paa |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
https://dx.doi.org/10.1111/j.1365-294x.2011.05424.x |
op_rights |
Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 |
op_doi |
https://doi.org/10.5061/dryad.443t4m1q10.1111/j.1365-294x.2011.05424.x |
_version_ |
1789972214549839872 |