Data from: Stress response or beneficial temperature acclimation: transcriptomic signatures in Antarctic fish (Pachycara brachycephalum) ...

• Main Authors: Windisch, Heidrun S., Frickenhaus, Stephan, John, Uwe, Knust, Rainer, Pörtner, Hans-Otto, Lucassen, Magnus
• Format: Dataset
• Language: English
• Published: Dryad 2014
• Subjects: cold-adaptation; Pachycara brachycephalum; microarray; Gene regulation; chronic thermal exposure; ESTs; cDNA library; Antarctic; The Antarctic; Antarc*
• Online Access: https://dx.doi.org/10.5061/dryad.40rk0; https://datadryad.org/stash/dataset/doi:10.5061/dryad.40rk0

Research on the thermal biology of Antarctic marine organisms has increased awareness of their vulnerability to climate change, as a flipside of their adaptation to life in the permanent cold and their limited capacity to acclimate to variable temperatures. Here, we employed a species–specific microarray of the Antarctic eelpout, Pachycara brachycephalum to identify long-term shifts in gene expression after 2 months of acclimation to six temperatures between -1°C and 9°C. Changes in cellular processes comprised signalling, post-translational modification, cytoskeleton remodelling, metabolic shifts and alterations in the transcription as well as translation machinery. The magnitude of transcriptomic responses paralleled the change in whole animal performance. Optimal growth at 3°C occurred at a minimum in gene expression changes indicative of a balanced steady state. The up–regulation of ribosomal transcripts at 5°C and above was accompanied by the transcriptomic activation of differential protein degradation ... : animal dataThis file contains growth and sampling data of fish under study as well as quality parameters of RNA samples that were used in the expression analysis.animal_data.txtPb_nr_annotated_Blast2GOThis file contains sequence annotations from the nr-database of all represented transcripts on the array. The file can be loaded using the open source software Blast2GO. (http://www.blast2go.com/b2ghome)Pb_swissProt_annotated_Blast2GOThis file contains sequence annotations from the SwissProt-database of all represented transcripts on the array. The file can be loaded using the open source software Blast2GO. (http://www.blast2go.com/b2ghome) ...