Data from: Dysregulation of valvular interstitial cell let-7c, miR-17, miR-20a, and miR-30d in naturally occurring canine myxomatous mitral valve disease ...
Canine myxomatous mitral valve disease (MMVD) resembles the early stages of myxomatous pathology seen in human non-syndromic mitral valve prolapse, a common valvular heart disease in the adult human population. Canine MMVD is seen in older subjects, suggesting age-related epigenetic dysregulation le...
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ftdatacite:10.5061/dryad.3274k 2024-10-13T14:06:33+00:00 Data from: Dysregulation of valvular interstitial cell let-7c, miR-17, miR-20a, and miR-30d in naturally occurring canine myxomatous mitral valve disease ... Yang, Vicky K. Tai, Albert K. Huh, Terry P. Meola, Dawn M. Juhr, Christine M. Robinson, Nicholas A. Hoffman, Andrew M. 2018 https://dx.doi.org/10.5061/dryad.3274k https://datadryad.org/stash/dataset/doi:10.5061/dryad.3274k en eng Dryad https://dx.doi.org/10.1371/journal.pone.0188617 Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 Canis lupus familiaris Dataset dataset 2018 ftdatacite https://doi.org/10.5061/dryad.3274k10.1371/journal.pone.0188617 2024-10-01T11:13:55Z Canine myxomatous mitral valve disease (MMVD) resembles the early stages of myxomatous pathology seen in human non-syndromic mitral valve prolapse, a common valvular heart disease in the adult human population. Canine MMVD is seen in older subjects, suggesting age-related epigenetic dysregulation leading to derangements in valvular cell populations and matrix synthesis or degradation. We hypothesized that valvular interstitial cells (VICs) undergo disease-relevant changes in miRNA expression. In primary VIC lines from diseased and control valves, miRNA expression was profiled using RT-qPCR and next generation sequencing. VICs from diseased valves showed phenotypic changes consistent with myofibroblastic differentiation (vimentinlow+, a-SMAhigh+), increases in senescence markers (p21, SA-b-gal), and decreased cell viability and proliferation potential. RT-qPCR and miRNA sequencing analyses both showed significant (p<0.05) downregulation of let-7c, miR-17, miR-20a, and miR-30d in VICs from diseased valves ... : RNAseq dataExcel data file with RNAseq count dataDESeq2_normalized_data.xlsxRT-qPCR DataPCR data with Ct numbersVICs PCR Data.xlsx ... Dataset Canis lupus DataCite |
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English |
topic |
Canis lupus familiaris |
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Canis lupus familiaris Yang, Vicky K. Tai, Albert K. Huh, Terry P. Meola, Dawn M. Juhr, Christine M. Robinson, Nicholas A. Hoffman, Andrew M. Data from: Dysregulation of valvular interstitial cell let-7c, miR-17, miR-20a, and miR-30d in naturally occurring canine myxomatous mitral valve disease ... |
topic_facet |
Canis lupus familiaris |
description |
Canine myxomatous mitral valve disease (MMVD) resembles the early stages of myxomatous pathology seen in human non-syndromic mitral valve prolapse, a common valvular heart disease in the adult human population. Canine MMVD is seen in older subjects, suggesting age-related epigenetic dysregulation leading to derangements in valvular cell populations and matrix synthesis or degradation. We hypothesized that valvular interstitial cells (VICs) undergo disease-relevant changes in miRNA expression. In primary VIC lines from diseased and control valves, miRNA expression was profiled using RT-qPCR and next generation sequencing. VICs from diseased valves showed phenotypic changes consistent with myofibroblastic differentiation (vimentinlow+, a-SMAhigh+), increases in senescence markers (p21, SA-b-gal), and decreased cell viability and proliferation potential. RT-qPCR and miRNA sequencing analyses both showed significant (p<0.05) downregulation of let-7c, miR-17, miR-20a, and miR-30d in VICs from diseased valves ... : RNAseq dataExcel data file with RNAseq count dataDESeq2_normalized_data.xlsxRT-qPCR DataPCR data with Ct numbersVICs PCR Data.xlsx ... |
format |
Dataset |
author |
Yang, Vicky K. Tai, Albert K. Huh, Terry P. Meola, Dawn M. Juhr, Christine M. Robinson, Nicholas A. Hoffman, Andrew M. |
author_facet |
Yang, Vicky K. Tai, Albert K. Huh, Terry P. Meola, Dawn M. Juhr, Christine M. Robinson, Nicholas A. Hoffman, Andrew M. |
author_sort |
Yang, Vicky K. |
title |
Data from: Dysregulation of valvular interstitial cell let-7c, miR-17, miR-20a, and miR-30d in naturally occurring canine myxomatous mitral valve disease ... |
title_short |
Data from: Dysregulation of valvular interstitial cell let-7c, miR-17, miR-20a, and miR-30d in naturally occurring canine myxomatous mitral valve disease ... |
title_full |
Data from: Dysregulation of valvular interstitial cell let-7c, miR-17, miR-20a, and miR-30d in naturally occurring canine myxomatous mitral valve disease ... |
title_fullStr |
Data from: Dysregulation of valvular interstitial cell let-7c, miR-17, miR-20a, and miR-30d in naturally occurring canine myxomatous mitral valve disease ... |
title_full_unstemmed |
Data from: Dysregulation of valvular interstitial cell let-7c, miR-17, miR-20a, and miR-30d in naturally occurring canine myxomatous mitral valve disease ... |
title_sort |
data from: dysregulation of valvular interstitial cell let-7c, mir-17, mir-20a, and mir-30d in naturally occurring canine myxomatous mitral valve disease ... |
publisher |
Dryad |
publishDate |
2018 |
url |
https://dx.doi.org/10.5061/dryad.3274k https://datadryad.org/stash/dataset/doi:10.5061/dryad.3274k |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
https://dx.doi.org/10.1371/journal.pone.0188617 |
op_rights |
Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 |
op_doi |
https://doi.org/10.5061/dryad.3274k10.1371/journal.pone.0188617 |
_version_ |
1812812738746908672 |