Aquaculture-driven evolution of the salmon louse mtDNA genome ...

• Main Author: Hasti Rutle, Karoline
• Format: Dataset
• Language: English
• Published: Dryad 2023
• Subjects: FOS Biological sciences; Lepeophtheirus salmonis; deltamethrin; Aquaculture; Atlantic salmon; North Atlantic
• Online Access: https://dx.doi.org/10.5061/dryad.2jm63xsvd; https://datadryad.org/stash/dataset/doi:10.5061/dryad.2jm63xsvd

Resistance towards the antiparasitic pyrethroid, deltamethrin, is reported in the Atlantic salmon louse (Lepeophtheirus salmonis salmonis), a persistent ectoparasite of farmed and wild salmonids. The resistance mechanism is linked to mitochondrial DNA (mtDNA), where genetic markers for resistance have been identified. Here, we investigated how widespread pyrethroid use in aquaculture may have influenced mtDNA variation in lice, and the dispersion of resistant haplotypes across the North Atlantic, using historical (2000–2002 “pre-resistance”) and contemporary (2014–2017 “post-resistance”) samples. To study this, we sequenced ATPase 6 and cytochrome b, genotyped two genetic markers for deltamethrin resistance, and genotyped microsatellites as “neutral” controls of potential population bottlenecks. Overall, we observed a modest reduction in mtDNA diversity in the period 2000–2017, but no reduction in microsatellite variation was observed. The reduction in mtDNA variation was especially distinct in two of the ... : The genotyped resistance data for deltamethrin and organophosphate of salmon lice collected in the Northern Atlantic during the time frame of 2014 to 2017, combined with the mitochondrial sequences using Sanger sequencing technique for both the mitochondrial genes ATPase 6 and Cytochrome b are available in the Excel file Resistance_mitochondrial_data.xls. In addition, microsatellite genotypes of salmon lice collected in the time frame 2000 to 2017 in the Northern Atlantic, are available in the Excel file Microsatellite_data.xls. ...