Environmental DNA detection of Arctic char (Salvelinus alpinus) in Irish lakes: development and application of a species-specific molecular assay ...
Populations of Arctic char (Salvelinus alpinus) inhabiting Irish lakes are undergoing a rapid decline. In order to aid monitoring of this species in Irish lakes, a species/specific qPCR assay was designed and subsequently tested for the detection of Arctic char’s environmental DNA (eDNA) in an indoo...
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Online Access: | https://dx.doi.org/10.5061/dryad.2280gb5nd https://datadryad.org/stash/dataset/doi:10.5061/dryad.2280gb5nd |
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ftdatacite:10.5061/dryad.2280gb5nd 2024-02-04T09:57:16+01:00 Environmental DNA detection of Arctic char (Salvelinus alpinus) in Irish lakes: development and application of a species-specific molecular assay ... Mirimin, Luca 2019 https://dx.doi.org/10.5061/dryad.2280gb5nd https://datadryad.org/stash/dataset/doi:10.5061/dryad.2280gb5nd en eng Dryad Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 Dataset dataset 2019 ftdatacite https://doi.org/10.5061/dryad.2280gb5nd 2024-01-05T00:42:33Z Populations of Arctic char (Salvelinus alpinus) inhabiting Irish lakes are undergoing a rapid decline. In order to aid monitoring of this species in Irish lakes, a species/specific qPCR assay was designed and subsequently tested for the detection of Arctic char’s environmental DNA (eDNA) in an indoor mesocosm as well as in natural habitats (six lakes). Specifically, the mesocosm experiment revealed that sample volume can have significant effects on the application of eDNA approaches, whereby probability of detection depends from proximity to the source organism as well as volume of water filtered. When tested in the wild, positive amplification of Arctic char eDNA was observed in four of the six lakes sampled, proving that this approach can be successfully implemented in natural conditions. However, findings from the present study also showed that sub-optimal sampling strategies can lead to false negative results (e.g. failure to detect the species when it is present). This study corroborates findings from ... Dataset Arctic Salvelinus alpinus DataCite Metadata Store (German National Library of Science and Technology) Arctic |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
description |
Populations of Arctic char (Salvelinus alpinus) inhabiting Irish lakes are undergoing a rapid decline. In order to aid monitoring of this species in Irish lakes, a species/specific qPCR assay was designed and subsequently tested for the detection of Arctic char’s environmental DNA (eDNA) in an indoor mesocosm as well as in natural habitats (six lakes). Specifically, the mesocosm experiment revealed that sample volume can have significant effects on the application of eDNA approaches, whereby probability of detection depends from proximity to the source organism as well as volume of water filtered. When tested in the wild, positive amplification of Arctic char eDNA was observed in four of the six lakes sampled, proving that this approach can be successfully implemented in natural conditions. However, findings from the present study also showed that sub-optimal sampling strategies can lead to false negative results (e.g. failure to detect the species when it is present). This study corroborates findings from ... |
format |
Dataset |
author |
Mirimin, Luca |
spellingShingle |
Mirimin, Luca Environmental DNA detection of Arctic char (Salvelinus alpinus) in Irish lakes: development and application of a species-specific molecular assay ... |
author_facet |
Mirimin, Luca |
author_sort |
Mirimin, Luca |
title |
Environmental DNA detection of Arctic char (Salvelinus alpinus) in Irish lakes: development and application of a species-specific molecular assay ... |
title_short |
Environmental DNA detection of Arctic char (Salvelinus alpinus) in Irish lakes: development and application of a species-specific molecular assay ... |
title_full |
Environmental DNA detection of Arctic char (Salvelinus alpinus) in Irish lakes: development and application of a species-specific molecular assay ... |
title_fullStr |
Environmental DNA detection of Arctic char (Salvelinus alpinus) in Irish lakes: development and application of a species-specific molecular assay ... |
title_full_unstemmed |
Environmental DNA detection of Arctic char (Salvelinus alpinus) in Irish lakes: development and application of a species-specific molecular assay ... |
title_sort |
environmental dna detection of arctic char (salvelinus alpinus) in irish lakes: development and application of a species-specific molecular assay ... |
publisher |
Dryad |
publishDate |
2019 |
url |
https://dx.doi.org/10.5061/dryad.2280gb5nd https://datadryad.org/stash/dataset/doi:10.5061/dryad.2280gb5nd |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Salvelinus alpinus |
genre_facet |
Arctic Salvelinus alpinus |
op_rights |
Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 |
op_doi |
https://doi.org/10.5061/dryad.2280gb5nd |
_version_ |
1789961586585108480 |