Data from: Adélie penguin population diet monitoring by analysis of food DNA in scats ...

• Main Authors: Jarman, Simon N., McInnes, Julie C., Faux, Cassandra, Polanowski, Andrea M., Marthick, James, Deagle, Bruce E., Southwell, Colin, Emmerson, Louise
• Format: Dataset
• Language: English
• Published: Dryad 2014
• Subjects: CCAMLR Ecosystem Monitoring; scat analysis; Euphausiidae; Pygoscelis adeliae; Adélie penguin; Ion Torrent Amplicon Sequencing; Southern Ocean
• Online Access: https://dx.doi.org/10.5061/dryad.1rf7d; https://datadryad.org/stash/dataset/doi:10.5061/dryad.1rf7d

The Adélie penguin is the most important animal currently used for ecosystem monitoring in the Southern Ocean. The diet of this species is generally studied by visual analysis of stomach contents; or ratios of isotopes of carbon and nitrogen incorporated into the penguin from its food. There are significant limitations to the information that can be gained from these methods. We evaluated population diet assessment by analysis of food DNA in scats as an alternative method for ecosystem monitoring with Adélie penguins as an indicator species. Scats were collected at four locations, three phases of the breeding cycle, and in four different years. A novel molecular diet assay and bioinformatics pipeline based on nuclear small subunit ribosomal RNA gene (SSU rDNA) sequencing was used to identify prey DNA in 389 scats. Analysis of the twelve population sample sets identified spatial and temporal dietary change in Adélie penguin population diet. Prey diversity was found to be greater than previously thought. ... : 1_AdelieDietPopulationResults2_FASTQfiles3_SampleKeyFiles4_SSUdietPipeline ...