Data from: Diversity among rare and common congeneric plant species from the Garry oak and Okanagan shrub-steppe ecosystems in British Columbia: implications for conservation ...
Using universal non-coding chloroplast DNA markers (cpDNA), we investigated genetic diversity and genetic structure in four rare and common plant species pairs inhabiting threatened ecosystems (Garry Oak and Okanagan shrub-steppe) in British Columbia. The species found in the Garry oak ecosystem are...
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Online Access: | https://dx.doi.org/10.5061/dryad.12jm63z1h https://datadryad.org/stash/dataset/doi:10.5061/dryad.12jm63z1h |
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ftdatacite:10.5061/dryad.12jm63z1h 2024-02-04T10:01:19+01:00 Data from: Diversity among rare and common congeneric plant species from the Garry oak and Okanagan shrub-steppe ecosystems in British Columbia: implications for conservation ... Hersh, Evan Wheeler, Erica Costanzo, Brenda Ramakrishnan, Alisa Miscampbell, Allyson Ritland, Carol Whitton, Jeannette Gorrell, Jamieson Harrower, William 2022 https://dx.doi.org/10.5061/dryad.12jm63z1h https://datadryad.org/stash/dataset/doi:10.5061/dryad.12jm63z1h en eng Dryad https://dx.doi.org/10.5281/zenodo.6829059 Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 FOS Biological sciences Conservation genetics British Columbia rare plants Chloroplast DNA Dataset dataset 2022 ftdatacite https://doi.org/10.5061/dryad.12jm63z1h10.5281/zenodo.6829059 2024-01-05T04:39:59Z Using universal non-coding chloroplast DNA markers (cpDNA), we investigated genetic diversity and genetic structure in four rare and common plant species pairs inhabiting threatened ecosystems (Garry Oak and Okanagan shrub-steppe) in British Columbia. The species found in the Garry oak ecosystem are: Sanicula bipinnatifida (purple sanicle; Apiaceae; rare), Sanicula crassicaulis (Pacific sanicle; Apiaceae; common), and Balsamorhiza deltoidea (deltoid balsamroot; Asteraceae; rare). The species found in the Okanagan shrub-steppe ecosystem are: Balsamorhiza sagittata (arrowleaf balsamroot; Asteraceae; common), Orthocarpus barbatus (Grand Coulee owl-clover; Orobanchaceae; rare), Orthocarpus luteus (yellow owl-clover; Orobanchaceae; common), Phacelia ramosissima (branching phacelia; Hydrophyllaceae; rare), and Phacelia linearis (thread-leaved phacelia; Hydrophyllaceae; common). Eight cpDNA regions were sequenced for each study species. Sequences were aligned and concatenated within each species, and single ... : Sample Collection We sampled single individuals from a total of 95 populations. Seventy-three samples were leaf excisions, taken with permission from herbarium specimens representing populations in western North America including the provinces/states British Columbia, California, Idaho, Oregon, Nevada, and Washington. In addition, we collected 22 fresh leaf samples from well-documented and previously vouchered populations of rare species in regional, provincial, and national parks in British Columbia. We placed fresh leaf tissue in paper envelopes inside a sealed plastic bag containing silica gel desiccant and stored them at room temperature. We assigned each of the 95 samples to northern or southern regional categories based on the location of the population sampled in relation to the last glacial maximum (LGM) of the Cordilleran ice sheet. DNA extraction and sequencing We ground 10 mg of dried leaf tissue (herbarium) or lyophilized fresh tissue of each sample using a Qiagen Tissuelyser II (Qiagen, ... Dataset Ice Sheet DataCite Metadata Store (German National Library of Science and Technology) Garry ENVELOPE(-62.233,-62.233,-63.350,-63.350) Pacific |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
FOS Biological sciences Conservation genetics British Columbia rare plants Chloroplast DNA |
spellingShingle |
FOS Biological sciences Conservation genetics British Columbia rare plants Chloroplast DNA Hersh, Evan Wheeler, Erica Costanzo, Brenda Ramakrishnan, Alisa Miscampbell, Allyson Ritland, Carol Whitton, Jeannette Gorrell, Jamieson Harrower, William Data from: Diversity among rare and common congeneric plant species from the Garry oak and Okanagan shrub-steppe ecosystems in British Columbia: implications for conservation ... |
topic_facet |
FOS Biological sciences Conservation genetics British Columbia rare plants Chloroplast DNA |
description |
Using universal non-coding chloroplast DNA markers (cpDNA), we investigated genetic diversity and genetic structure in four rare and common plant species pairs inhabiting threatened ecosystems (Garry Oak and Okanagan shrub-steppe) in British Columbia. The species found in the Garry oak ecosystem are: Sanicula bipinnatifida (purple sanicle; Apiaceae; rare), Sanicula crassicaulis (Pacific sanicle; Apiaceae; common), and Balsamorhiza deltoidea (deltoid balsamroot; Asteraceae; rare). The species found in the Okanagan shrub-steppe ecosystem are: Balsamorhiza sagittata (arrowleaf balsamroot; Asteraceae; common), Orthocarpus barbatus (Grand Coulee owl-clover; Orobanchaceae; rare), Orthocarpus luteus (yellow owl-clover; Orobanchaceae; common), Phacelia ramosissima (branching phacelia; Hydrophyllaceae; rare), and Phacelia linearis (thread-leaved phacelia; Hydrophyllaceae; common). Eight cpDNA regions were sequenced for each study species. Sequences were aligned and concatenated within each species, and single ... : Sample Collection We sampled single individuals from a total of 95 populations. Seventy-three samples were leaf excisions, taken with permission from herbarium specimens representing populations in western North America including the provinces/states British Columbia, California, Idaho, Oregon, Nevada, and Washington. In addition, we collected 22 fresh leaf samples from well-documented and previously vouchered populations of rare species in regional, provincial, and national parks in British Columbia. We placed fresh leaf tissue in paper envelopes inside a sealed plastic bag containing silica gel desiccant and stored them at room temperature. We assigned each of the 95 samples to northern or southern regional categories based on the location of the population sampled in relation to the last glacial maximum (LGM) of the Cordilleran ice sheet. DNA extraction and sequencing We ground 10 mg of dried leaf tissue (herbarium) or lyophilized fresh tissue of each sample using a Qiagen Tissuelyser II (Qiagen, ... |
format |
Dataset |
author |
Hersh, Evan Wheeler, Erica Costanzo, Brenda Ramakrishnan, Alisa Miscampbell, Allyson Ritland, Carol Whitton, Jeannette Gorrell, Jamieson Harrower, William |
author_facet |
Hersh, Evan Wheeler, Erica Costanzo, Brenda Ramakrishnan, Alisa Miscampbell, Allyson Ritland, Carol Whitton, Jeannette Gorrell, Jamieson Harrower, William |
author_sort |
Hersh, Evan |
title |
Data from: Diversity among rare and common congeneric plant species from the Garry oak and Okanagan shrub-steppe ecosystems in British Columbia: implications for conservation ... |
title_short |
Data from: Diversity among rare and common congeneric plant species from the Garry oak and Okanagan shrub-steppe ecosystems in British Columbia: implications for conservation ... |
title_full |
Data from: Diversity among rare and common congeneric plant species from the Garry oak and Okanagan shrub-steppe ecosystems in British Columbia: implications for conservation ... |
title_fullStr |
Data from: Diversity among rare and common congeneric plant species from the Garry oak and Okanagan shrub-steppe ecosystems in British Columbia: implications for conservation ... |
title_full_unstemmed |
Data from: Diversity among rare and common congeneric plant species from the Garry oak and Okanagan shrub-steppe ecosystems in British Columbia: implications for conservation ... |
title_sort |
data from: diversity among rare and common congeneric plant species from the garry oak and okanagan shrub-steppe ecosystems in british columbia: implications for conservation ... |
publisher |
Dryad |
publishDate |
2022 |
url |
https://dx.doi.org/10.5061/dryad.12jm63z1h https://datadryad.org/stash/dataset/doi:10.5061/dryad.12jm63z1h |
long_lat |
ENVELOPE(-62.233,-62.233,-63.350,-63.350) |
geographic |
Garry Pacific |
geographic_facet |
Garry Pacific |
genre |
Ice Sheet |
genre_facet |
Ice Sheet |
op_relation |
https://dx.doi.org/10.5281/zenodo.6829059 |
op_rights |
Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 |
op_doi |
https://doi.org/10.5061/dryad.12jm63z1h10.5281/zenodo.6829059 |
_version_ |
1789967094310240256 |