Data from: Diversity among rare and common congeneric plant species from the Garry oak and Okanagan shrub-steppe ecosystems in British Columbia: implications for conservation ...

• Main Authors: Hersh, Evan, Wheeler, Erica, Costanzo, Brenda, Ramakrishnan, Alisa, Miscampbell, Allyson, Ritland, Carol, Whitton, Jeannette, Gorrell, Jamieson, Harrower, William
• Format: Dataset
• Language: English
• Published: Dryad 2022
• Subjects: FOS Biological sciences; Conservation genetics; British Columbia; rare plants; Chloroplast DNA; Garry; Pacific; Ice Sheet
• Online Access: https://dx.doi.org/10.5061/dryad.12jm63z1h; https://datadryad.org/stash/dataset/doi:10.5061/dryad.12jm63z1h

Using universal non-coding chloroplast DNA markers (cpDNA), we investigated genetic diversity and genetic structure in four rare and common plant species pairs inhabiting threatened ecosystems (Garry Oak and Okanagan shrub-steppe) in British Columbia. The species found in the Garry oak ecosystem are: Sanicula bipinnatifida (purple sanicle; Apiaceae; rare), Sanicula crassicaulis (Pacific sanicle; Apiaceae; common), and Balsamorhiza deltoidea (deltoid balsamroot; Asteraceae; rare). The species found in the Okanagan shrub-steppe ecosystem are: Balsamorhiza sagittata (arrowleaf balsamroot; Asteraceae; common), Orthocarpus barbatus (Grand Coulee owl-clover; Orobanchaceae; rare), Orthocarpus luteus (yellow owl-clover; Orobanchaceae; common), Phacelia ramosissima (branching phacelia; Hydrophyllaceae; rare), and Phacelia linearis (thread-leaved phacelia; Hydrophyllaceae; common). Eight cpDNA regions were sequenced for each study species. Sequences were aligned and concatenated within each species, and single ... : Sample Collection We sampled single individuals from a total of 95 populations. Seventy-three samples were leaf excisions, taken with permission from herbarium specimens representing populations in western North America including the provinces/states British Columbia, California, Idaho, Oregon, Nevada, and Washington. In addition, we collected 22 fresh leaf samples from well-documented and previously vouchered populations of rare species in regional, provincial, and national parks in British Columbia. We placed fresh leaf tissue in paper envelopes inside a sealed plastic bag containing silica gel desiccant and stored them at room temperature. We assigned each of the 95 samples to northern or southern regional categories based on the location of the population sampled in relation to the last glacial maximum (LGM) of the Cordilleran ice sheet. DNA extraction and sequencing We ground 10 mg of dried leaf tissue (herbarium) or lyophilized fresh tissue of each sample using a Qiagen Tissuelyser II (Qiagen, ...