Files for phylogenetics, structure, and migration rate analyses for the bivalve Aequiyoldia eightsii ...

• Main Authors: Muñoz-Ramírez, Carlos P., Sands, Chester, Barnes, David, Scourse, James, Roman-Gonzalez, Alejandro, Morley, Simon, Cardenas, Leyla, Brante, Antonio, Meredith, Michael
• Format: Dataset
• Language: English
• Published: Dryad 2019
• Subjects: nextRAD; Ocean currents; West Antarctic Peninsula; bivalve; Antarctic Peninsula Coastal Current; Antarctic; Antarctic Peninsula; Southern Ocean; The Antarctic; Antarc*
• Online Access: https://dx.doi.org/10.5061/dryad.0cfxpnvxr; https://datadryad.org/stash/dataset/doi:10.5061/dryad.0cfxpnvxr

The Antarctic Circumpolar Current (ACC) dominates the open-ocean circulation of the Southern Ocean, and both isolates and connects the Southern Ocean biodiversity. However, the impact on biological processes of other Southern Ocean currents is less clear. Adjacent to the West Antarctic Peninsula (WAP), the ACC flows offshore in a northeastward direction, whereas the Antarctic Peninsula Coastal Current (APCC) follows a complex circulation pattern along the coast, with topographically-influenced deflections depending on the area. Using genomic data, we estimated genetic structure and migration rates between populations of the benthic bivalve Aequiyoldia eightsii from the shallows of southern South America and the WAP to test the role of the ACC and the APCC in its dispersal. We found strong genetic structure across the ACC (between southern South America and Antarctica) and moderate structure between populations of the West Antarctic Peninsula. Migration rates along the WAP were consistent with the APCC being ... : Genomic DNA was extracted with the Qiagen DNeasy Blood & Tissue kit, following the manufacturer's protocol. Mitochondrial DNA data were collected to conduct a preliminary assessment of phylogeographic patterns and to confirm a single evolutionary lineage, given that multiple lineages have been documented previously [12]. A 629 bp fragment of the cytochrome c oxidase subunit I (COI) gene was amplified using universal primers from [17] with final concentrations for PCR components per 25 μL reaction as follows: 25 ng template DNA, 0.25 μM of each primer, 0.625 units of GoTaq DNA polymerase (Promega, Madison, WI, USA), 0.1 mM of each dNTP, 2.5 μL of 10 reaction buffer and 2.5 mM MgCl2. Amplification parameters were as follows: 95 °C for 2 min followed by 35 cycles of 95 °C for 30 s, 48 °C for 30 s, 72 °C for 90 s, and 72 °C for 7 min. Purification and sequencing were conducted at MACROGEN Inc. (South Korea). Chromatograms were edited in CodonCode Aligner 8.0.2 (Dedham, MA, USA). Sequences were imported to ...