Pycnophyidae COX1 and ITS sequences from Disko Island, Greenland and Svalbard ...
Material for the present study was collected during two sampling campaigns: 1. to Disko Island (Western Greenland), organised by the Natural History Museum of Denmark (NHMD) in August 3-9, 2023 covering twelve sampling stations; 2. to Western Spitsbergen fjords between 5 and 25 of August 2024, organ...
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| Format: | Dataset |
| Language: | English |
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Institute of Oceanology Polish Academy of Sciences
2025
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| Online Access: | https://dx.doi.org/10.48457/iopan.2025.532 https://geonetwork.iopan.pl/geonetwork/srv/resources/records/87fc16e7-4153-4779-a8c7-2eaa49adc74f |
| Summary: | Material for the present study was collected during two sampling campaigns: 1. to Disko Island (Western Greenland), organised by the Natural History Museum of Denmark (NHMD) in August 3-9, 2023 covering twelve sampling stations; 2. to Western Spitsbergen fjords between 5 and 25 of August 2024, organised by IOPAN as a part of the annual scientific expedition on board of r/v Oceania. Sediment samples were collected with a dredge and/or a Van Veen grab. Kinorhynchs were extracted from the sediment, sorted under a stereomicroscope, preserved in 99 % EtOH and kept in −20 ◦C until further analysis. Single individuals were selected for DNA extraction using Tissue DNA Purification Kit (EURx, Gdansk, Poland) with additional step of cuticle retention (centrifugation of lysis buffer at 11,000×g, followed by separation of exoskeleton from supernatant including eluted DNA). The exoskeletons were recovered, transferred to a microscopic glass slide and mounted in Fluoromount-G for light microscopy (LM). The exoskeletons ... |
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