Kinorhyncha COX1 sequences from Disko Island, Greenland ...

• Main Author: Zalewska, Aleksandra
• Format: Dataset
• Language: English
• Published: Institute of Oceanology Polish Academy of Sciences 2024
• Subjects: Greenland; molecular biology parameters; Olympus; Van Veen
• Online Access: https://dx.doi.org/10.48457/iopan.2025.503; https://geonetwork.iopan.pl/geonetwork/srv/resources/records/c6f12360-235a-464d-af8f-baaa179d4e0b

Material for the present study was collected from twelve sampling stations in August 3-9, 2023, during an expedition to Disko Island (Western Greenland), organised by the Natural History Museum of Denmark (NHMD). Sediment samples were collected with a dredge and/or a Van Veen grab. Kinorhynchs were extracted from the sediment, sorted under a stereomicroscope, preserved in 99 % EtOH and kept in −20 ◦C until further analysis. Single individuals were selected for DNA extraction using Tissue DNA Purification Kit (EURx, Gdansk, Poland) with additional step of cuticle retention (centrifugation of lysis buffer at 11,000×g, followed by separation of exoskeleton from supernatant including eluted DNA). The exoskeletons were recovered, transferred to a microscopic glass slide and mounted in Fluoromount-G for light microscopy (LM). The exoskeletons were examined using a Nikon 600 and an Olympus BX51 equipped with an Olympus SC300 camera, catalogued and deposited in NHMD. DNA samples were used for fragment cox1 gene PCR ...