Innate antiviral-like immune responses differ among divergent European and North American farmed and wild Atlantic salmon (Salmo salar) and their hybrids ...

• Main Author: Dove, Sindy
• Format: Text
• Language: English
• Published: Memorial University of Newfoundland 2022
• Subjects: Atlantic salmon; Newfoundland; Salmo salar
• Online Access: https://dx.doi.org/10.48336/mjf8-de58; https://research.library.mun.ca/15720/

This thesis involved a study of the effect of North American (NA) farmed, European origin (EO) farmed, wild, and related hybrid cross types on the innate antiviral-like immune response in Atlantic salmon (Salmo salar). Since the 1970s, millions of farmed salmon have escaped causing concern about how interbreeding with wild salmon may affect wild populations. Farmed and wild salmon differ genetically, yet the immune responses of farmed-wild hybrids are not well understood. In Newfoundland, permission has been granted to start farming an EO strain in addition to the current NA strain from the Saint John River. As these strains differ genetically and geographically, there is concern about potential differences in immune responses. Hence, I used real-time quantitative polymerase chain reaction (qPCR) with head kidney templates to measure expression of seventeen antiviral and other immune-relevant biomarker genes (i.e., dhx58, gig1a, helz2, ifna, ifng, irf1a, irf7b, isg15a, mxb, pgds, rsad2a, rsad2b, stat1a, ...