Transcriptomic studies of Atlantic salmon responses to dietary omega-3 long-chain polyunsaturated fatty acids, Piscirickettsia salmonis infection, immunogens, and functional feed ...

• Main Author: Xue, Xi
• Format: Text
• Language: English
• Published: Memorial University of Newfoundland 2022
• Subjects: Atlantic salmon
• Online Access: https://dx.doi.org/10.48336/fff8-0208; https://research.library.mun.ca/15871/

Atlantic salmon farming faces several issues and challenges such as disease outbreaks and the limited supply of fish oil, affecting the sustainability of the industry. The current thesis aimed to gain a better understanding of the dietary requirement of omega-3 long-chain polyunsaturated fatty acids (ω3 LC-PUFA; i.e. DHA+EPA), immune responses against Piscirickettsia salmonis or immunogens, and the immune-modulating properties of a functional feed in Atlantic salmon. In Chapter 2, I studied the impact of varying levels of DHA and EPA (0, 1.0, and 1.4% of the diet) on the hepatic transcriptome. My data suggested that ~1.0% of dietary ω3 LC-PUFA is sufficient to sustain the optimal growth performance of Atlantic salmon, but may not be enough to maintain good health and survival compared with fish fed diets containing a higher amount of DHA and EPA. In Chapter 3, Atlantic salmon parr were infected with an EM-90-like isolate of Piscirickettsia salmonis through intraperitoneal injection. I used microarray and ...