Molecular immune responses of lumpfish (Cyclopterus lumpus) to aeromonas salmonicida ...
Lumpfish (Cyclopterus lumpus) have been utilized to biocontrol sea-lice (e.g., Lepeophtheirus salmonis) infestations in Atlantic salmon (Salmo salar) farms in the North Atlantic region. Aeromonas salmonicida subsp. salmonicida is a Gram-negative facultative intracellular pathogen infecting several f...
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Memorial University of Newfoundland
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Online Access: | https://dx.doi.org/10.48336/771q-rk92 https://research.library.mun.ca/16247/ |
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ftdatacite:10.48336/771q-rk92 2023-12-31T10:04:47+01:00 Molecular immune responses of lumpfish (Cyclopterus lumpus) to aeromonas salmonicida ... Chakraborty, Setu 2023 https://dx.doi.org/10.48336/771q-rk92 https://research.library.mun.ca/16247/ en eng Memorial University of Newfoundland ScholarlyArticle article-journal Text 2023 ftdatacite https://doi.org/10.48336/771q-rk92 2023-12-01T11:44:14Z Lumpfish (Cyclopterus lumpus) have been utilized to biocontrol sea-lice (e.g., Lepeophtheirus salmonis) infestations in Atlantic salmon (Salmo salar) farms in the North Atlantic region. Aeromonas salmonicida subsp. salmonicida is a Gram-negative facultative intracellular pathogen infecting several fish species, including lumpfish. Here, I established an A. salmonicida systemic infection model in lumpfish. Juvenile lumpfish were intraperitoneally (ip) injected with different doses of A. salmonicida strain J223. A. salmonicida median lethal dose (LD50) was calculated at 102 colony-forming units (CFU)/dose. Samples from blood, head kidney, spleen, and liver were collected at different time points to determine the infection kinetics. Triplicated RNA samples from the head kidney, spleen, and liver of non-infected and infected (104 CFU/dose) lumpfish at 3 and 10 days post-infection (dpi) were utilized for RNA sequencing (RNA-Seq). Analysis of RNA-Seq data suggested that A. salmonicida could induce lethal infection ... Text Atlantic salmon North Atlantic Salmo salar DataCite Metadata Store (German National Library of Science and Technology) |
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English |
description |
Lumpfish (Cyclopterus lumpus) have been utilized to biocontrol sea-lice (e.g., Lepeophtheirus salmonis) infestations in Atlantic salmon (Salmo salar) farms in the North Atlantic region. Aeromonas salmonicida subsp. salmonicida is a Gram-negative facultative intracellular pathogen infecting several fish species, including lumpfish. Here, I established an A. salmonicida systemic infection model in lumpfish. Juvenile lumpfish were intraperitoneally (ip) injected with different doses of A. salmonicida strain J223. A. salmonicida median lethal dose (LD50) was calculated at 102 colony-forming units (CFU)/dose. Samples from blood, head kidney, spleen, and liver were collected at different time points to determine the infection kinetics. Triplicated RNA samples from the head kidney, spleen, and liver of non-infected and infected (104 CFU/dose) lumpfish at 3 and 10 days post-infection (dpi) were utilized for RNA sequencing (RNA-Seq). Analysis of RNA-Seq data suggested that A. salmonicida could induce lethal infection ... |
format |
Text |
author |
Chakraborty, Setu |
spellingShingle |
Chakraborty, Setu Molecular immune responses of lumpfish (Cyclopterus lumpus) to aeromonas salmonicida ... |
author_facet |
Chakraborty, Setu |
author_sort |
Chakraborty, Setu |
title |
Molecular immune responses of lumpfish (Cyclopterus lumpus) to aeromonas salmonicida ... |
title_short |
Molecular immune responses of lumpfish (Cyclopterus lumpus) to aeromonas salmonicida ... |
title_full |
Molecular immune responses of lumpfish (Cyclopterus lumpus) to aeromonas salmonicida ... |
title_fullStr |
Molecular immune responses of lumpfish (Cyclopterus lumpus) to aeromonas salmonicida ... |
title_full_unstemmed |
Molecular immune responses of lumpfish (Cyclopterus lumpus) to aeromonas salmonicida ... |
title_sort |
molecular immune responses of lumpfish (cyclopterus lumpus) to aeromonas salmonicida ... |
publisher |
Memorial University of Newfoundland |
publishDate |
2023 |
url |
https://dx.doi.org/10.48336/771q-rk92 https://research.library.mun.ca/16247/ |
genre |
Atlantic salmon North Atlantic Salmo salar |
genre_facet |
Atlantic salmon North Atlantic Salmo salar |
op_doi |
https://doi.org/10.48336/771q-rk92 |
_version_ |
1786836200737210368 |