Ribosomally derived lipopeptides containing distinct fatty acyl moieties ...

• Main Authors: Hubrich, Florian, Bösch, Nina M., Chepkirui, Clara, Morinaka, Brandon I., Rust, Michael, Gugger, Muriel, Robinson, Serina L., Vagstad, Anna L., Piel, Jörn
• Format: Article in Journal/Newspaper
• Language: English
• Published: ETH Zurich 2022
• Subjects: RiPPs; natural products; peptides; N-acylation; biosynthesis; Arctic; Arctic Ocean
• Online Access: https://dx.doi.org/10.3929/ethz-b-000529017; http://hdl.handle.net/20.500.11850/529017

Lipopeptides represent a large group of microbial natural products that include important antibacterial and antifungal drugs and some of the most-powerful known biosurfactants. The vast majority of lipopeptides comprise cyclic peptide backbones N-terminally equipped with various fatty acyl moieties. The known compounds of this type are biosynthesized by nonribosomal peptide synthetases, giant enzyme complexes that assemble their products in a non-gene-encoded manner. Here, we report the genome-guided discovery of ribosomally derived, fatty-acylated lipopeptides, termed selidamides. Heterologous reconstitution of three pathways, two from cyanobacteria and one from an arctic, ocean-derived alphaproteobacterium, allowed structural characterization of the probable natural products and suggest that selidamides are widespread over various bacterial phyla. The identified representatives feature cyclic peptide moieties and fatty acyl units attached to (hydroxy)ornithine or lysine side chains by maturases of the ... : Proceedings of the National Academy of Sciences of the United States of America, 119 (3) ...