Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions ...
Humpback whales, like other polar wildlife, accumulate persistent organic pollutants. In Southern hemisphere populations, hexachlorobenzene (HCB) dominates the contaminant profiles. HCB is linked to a variety of health effects and is classified as a group 2B carcinogen, but the mechanism of action i...
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Online Access: | https://dx.doi.org/10.3929/ethz-b-000387161 http://hdl.handle.net/20.500.11850/387161 |
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ftdatacite:10.3929/ethz-b-000387161 2024-04-28T08:23:22+00:00 Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions ... Maner, Jenny Burkard, Michael Cassano, Juan C. Bengtson Nash, Susan Schirmer, Kristin Suter, Marc J.-F. 2019 application/pdf https://dx.doi.org/10.3929/ethz-b-000387161 http://hdl.handle.net/20.500.11850/387161 en eng ETH Zurich info:eu-repo/semantics/openAccess Creative Commons Attribution Non Commercial 3.0 Unported https://creativecommons.org/licenses/by-nc/3.0/legalcode cc-by-nc-3.0 article-journal Text ScholarlyArticle Journal Article 2019 ftdatacite https://doi.org/10.3929/ethz-b-000387161 2024-04-02T12:34:54Z Humpback whales, like other polar wildlife, accumulate persistent organic pollutants. In Southern hemisphere populations, hexachlorobenzene (HCB) dominates the contaminant profiles. HCB is linked to a variety of health effects and is classified as a group 2B carcinogen, but the mechanism of action is a matter of contention. Potential toxicological effects to humpback whales remain entirely unknown. The recently established humpback whale fibroblast cell line (HuWa) offers an in vitro model for toxicological investigations. We here combine this novel cell line with a passive dosing strategy to investigate whale-specific toxicity of HCB. The relevant partitioning coefficients were determined to produce stable and predictable exposure concentrations in small-scale bioassays. The system was used to assess acute toxicity as well as genotoxicity of HCB to the HuWa cell line. While we found some transient reductions in metabolic activity, measured with the indicator dye alamarBlue, no clear acute toxic effects were ... : RSC Advances, 9 (67) ... Article in Journal/Newspaper Humpback Whale DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
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ftdatacite |
language |
English |
description |
Humpback whales, like other polar wildlife, accumulate persistent organic pollutants. In Southern hemisphere populations, hexachlorobenzene (HCB) dominates the contaminant profiles. HCB is linked to a variety of health effects and is classified as a group 2B carcinogen, but the mechanism of action is a matter of contention. Potential toxicological effects to humpback whales remain entirely unknown. The recently established humpback whale fibroblast cell line (HuWa) offers an in vitro model for toxicological investigations. We here combine this novel cell line with a passive dosing strategy to investigate whale-specific toxicity of HCB. The relevant partitioning coefficients were determined to produce stable and predictable exposure concentrations in small-scale bioassays. The system was used to assess acute toxicity as well as genotoxicity of HCB to the HuWa cell line. While we found some transient reductions in metabolic activity, measured with the indicator dye alamarBlue, no clear acute toxic effects were ... : RSC Advances, 9 (67) ... |
format |
Article in Journal/Newspaper |
author |
Maner, Jenny Burkard, Michael Cassano, Juan C. Bengtson Nash, Susan Schirmer, Kristin Suter, Marc J.-F. |
spellingShingle |
Maner, Jenny Burkard, Michael Cassano, Juan C. Bengtson Nash, Susan Schirmer, Kristin Suter, Marc J.-F. Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions ... |
author_facet |
Maner, Jenny Burkard, Michael Cassano, Juan C. Bengtson Nash, Susan Schirmer, Kristin Suter, Marc J.-F. |
author_sort |
Maner, Jenny |
title |
Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions ... |
title_short |
Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions ... |
title_full |
Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions ... |
title_fullStr |
Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions ... |
title_full_unstemmed |
Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions ... |
title_sort |
hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions ... |
publisher |
ETH Zurich |
publishDate |
2019 |
url |
https://dx.doi.org/10.3929/ethz-b-000387161 http://hdl.handle.net/20.500.11850/387161 |
genre |
Humpback Whale |
genre_facet |
Humpback Whale |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Attribution Non Commercial 3.0 Unported https://creativecommons.org/licenses/by-nc/3.0/legalcode cc-by-nc-3.0 |
op_doi |
https://doi.org/10.3929/ethz-b-000387161 |
_version_ |
1797584357064441856 |