An empirical method for absolute calibration of coccolith thickness ...
As major calcifiers in the open ocean, coccolithophores play a key role in the marine carbon cycle. Because they may be sensitive to changing CO2 and ocean acidification, there is significant interest in quantifying past and present variations in their cellular calcification by quantifying the thick...
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ftdatacite:10.3929/ethz-b-000248940 2024-04-28T08:34:50+00:00 An empirical method for absolute calibration of coccolith thickness ... González-Lemos, Saúl Guitián, José Fuertes, Miguel-Ángel Flores, José-Abel Stoll, Heather M. 2018 application/pdf https://dx.doi.org/10.3929/ethz-b-000248940 http://hdl.handle.net/20.500.11850/248940 en eng ETH Zurich info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 article-journal Text ScholarlyArticle Journal Article 2018 ftdatacite https://doi.org/10.3929/ethz-b-000248940 2024-04-02T12:33:25Z As major calcifiers in the open ocean, coccolithophores play a key role in the marine carbon cycle. Because they may be sensitive to changing CO2 and ocean acidification, there is significant interest in quantifying past and present variations in their cellular calcification by quantifying the thickness of the coccoliths or calcite plates that cover their cells. Polarized light microscopy has emerged as a key tool for quantifying the thickness of these calcite plates, but the reproducibility and accuracy of such determinations has been limited by the absence of suitable calibration materials in the thickness range of coccoliths (0–4 µm). Here, we describe the fabrication of a calcite wedge with a constant slope over this thickness range, and the independent determination of calcite thickness along the wedge profile. We show how the calcite wedge provides more robust calibrations in the 0 to 1.55 µm range than previous approaches using rhabdoliths. We show the particular advantages of the calcite wedge ... : Biogeosciences, 15 (4) ... Article in Journal/Newspaper Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
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ftdatacite |
language |
English |
description |
As major calcifiers in the open ocean, coccolithophores play a key role in the marine carbon cycle. Because they may be sensitive to changing CO2 and ocean acidification, there is significant interest in quantifying past and present variations in their cellular calcification by quantifying the thickness of the coccoliths or calcite plates that cover their cells. Polarized light microscopy has emerged as a key tool for quantifying the thickness of these calcite plates, but the reproducibility and accuracy of such determinations has been limited by the absence of suitable calibration materials in the thickness range of coccoliths (0–4 µm). Here, we describe the fabrication of a calcite wedge with a constant slope over this thickness range, and the independent determination of calcite thickness along the wedge profile. We show how the calcite wedge provides more robust calibrations in the 0 to 1.55 µm range than previous approaches using rhabdoliths. We show the particular advantages of the calcite wedge ... : Biogeosciences, 15 (4) ... |
format |
Article in Journal/Newspaper |
author |
González-Lemos, Saúl Guitián, José Fuertes, Miguel-Ángel Flores, José-Abel Stoll, Heather M. |
spellingShingle |
González-Lemos, Saúl Guitián, José Fuertes, Miguel-Ángel Flores, José-Abel Stoll, Heather M. An empirical method for absolute calibration of coccolith thickness ... |
author_facet |
González-Lemos, Saúl Guitián, José Fuertes, Miguel-Ángel Flores, José-Abel Stoll, Heather M. |
author_sort |
González-Lemos, Saúl |
title |
An empirical method for absolute calibration of coccolith thickness ... |
title_short |
An empirical method for absolute calibration of coccolith thickness ... |
title_full |
An empirical method for absolute calibration of coccolith thickness ... |
title_fullStr |
An empirical method for absolute calibration of coccolith thickness ... |
title_full_unstemmed |
An empirical method for absolute calibration of coccolith thickness ... |
title_sort |
empirical method for absolute calibration of coccolith thickness ... |
publisher |
ETH Zurich |
publishDate |
2018 |
url |
https://dx.doi.org/10.3929/ethz-b-000248940 http://hdl.handle.net/20.500.11850/248940 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_rights |
info:eu-repo/semantics/openAccess Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.3929/ethz-b-000248940 |
_version_ |
1797591368174927872 |