Chromosomal assignment of canine THADA gene to CFA 10q25 ...

• Main Authors: Soller, Jan T., Beuing, Claudia, Escobar, Hugo Murua, Winkler, Susanne, Reimann-Berg, Nicola, Drieschner, Norbert, Dolf, Gaudenz, Schelling, Claude, Nolte, Ingo, Bullerdiek, Jörn
• Format: Article in Journal/Newspaper
• Language: English
• Published: ETH Zurich 2008
• Subjects: Canis lupus
• Online Access: https://dx.doi.org/10.3929/ethz-b-000011660; http://hdl.handle.net/20.500.11850/11660

Background: Chromosomal translocations affecting the chromosome 2p21 cluster in a 450 kb breakpoint region are frequently observed in human benign thyroid adenomas. THADA (thyroid adenoma associated) was identified as the affected gene within this breakpoint region. In contrast to man tumours of the thyroid gland of dogs (Canis lupus familiaris) constitute mainly as follicular cell carcinomas, with malignant thyroid tumours being more frequent than benign thyroid adenomas. In order to elucidate if the THADA gene is also a target of chromosomal rearrangements in thyroid adenomas of the dog we have physically mapped the canine THADA gene to canine chromosome 10. A PCR was established to screen a canine genome library for a BAC clone containing the gene sequence of canine THADA. Further PCR reactions were done using the identified BAC clone as a template in order to verify the corresponding PCR product by sequencing. Canine whole blood was incubated with colcemid in order to arrest the cultured cells in ... : Molecular Cytogenetics, 1 ...