Strong signatures of selection in Atlantic herring: A marine fish with huge population sizes
Here, we present a cost-effective genome- wide study in a species that lacks a genome sequence. We first assembled a muscle transcriptome and then aligned genomic reads to the transcripts, creating an “exome assembly”, capturing both exons and flanking sequences. We then resequenced pools of fish fr...
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| Format: | Dataset |
| Language: | English |
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CNGB
2012
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| Online Access: | https://dx.doi.org/10.26036/cnphis0001092 https://db.cngb.org/search/project/PRJNA179109/ |
| Summary: | Here, we present a cost-effective genome- wide study in a species that lacks a genome sequence. We first assembled a muscle transcriptome and then aligned genomic reads to the transcripts, creating an “exome assembly”, capturing both exons and flanking sequences. We then resequenced pools of fish from a wide geographic range, including the Northeast Atlantic, as well as different regions in the Baltic Sea, aligned the reads to the exome assembly, and identified 440,817 SNPs. The great majority of SNPs showed no appreciable differences in allele frequency among populations; however, several thousand SNPs showed striking differences, some approaching fixation for different alleles. The contrast between low genetic differentiation at most loci and striking differences at others implies that the latter category primarily reflects natural selection. A simulation study confirmed that the distribution of the fixation index (FST) deviated significantly from expectation for selectively neutral loci. |
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