Gene expression profiles for Neocalanus flemingeri pre adults (CV) exposed to four different experimental food conditions collected from the M/V Dora in the Gulf of Alaska at station GAK1 from April 2019 ...

• Main Authors: Lenz, Petra H., Hartline, Daniel K., Roncalli, Vittoria, Block, Lauren N, Niestroy, Jeanette L., Cieslak, Matthew C.
• Format: Dataset
• Language: English
• Published: Biological and Chemical Oceanography Data Management Office (BCO-DMO) 2024
• Subjects: Gulf of Alaska; Neocalanus flemingeri; incubation experiment; lipid accumulation; gene expression; Alaska; Copepods
• Online Access: https://dx.doi.org/10.26008/1912/bco-dmo.914459.1; https://hdl.handle.net/1912/69767

This experimental dataset includes relative expression of individual Neocalanus flemingeri stage CV individuals incubated for different lengths of time and four different food treatments. The experimental protocol and results are described in detail in Roncalli et al., 2023. Briefly, field-collected N. flemingeri were allowed to molt into stage CV and then sorted into four different treatments: no food, low carbon, high carbon and high carbon with diatoms. After a one-week incubation, individuals from all four treatments were processed individually for RNA-Seq. In addition, following two and three-week incubations, copepods from the three fed treatments were processed individually for RNA-Seq. Short-sequence reads were mapped against a reference transcriptome and normalized gene expression was computed for each transcript. The dataset includes log-transformed relative gene expression in reads per kilobase per million reads (RPKM) (log2[RPKM+1]). The dataset also includes a list of differentially expressed ...