Exopolysaccharide production by Antarctic marine bacteria ...

• Main Author: Nichols, CAM
• Format: Thesis
• Language: unknown
• Published: University Of Tasmania 2023
• Subjects: Antarctic; Southern Ocean; Antarc*; Sea ice
• Online Access: https://dx.doi.org/10.25959/23246510.v1; https://figshare.utas.edu.au/articles/thesis/Exopolysaccharide_production_by_Antarctic_marine_bacteria/23246510/1

Antarctic marine bacteria isolated from sea ice and Southern Ocean particulate material were screened for exopolysaccharide (EPS) production. Ten strains were characterized using phenotypic (morphology), chemotaxonomic (whole cell fatty acid profiles) and phylogenetic (16S rDNA sequencing) techniques. These isolates were representatives of four genera including Pseudoalteromonas, Shewanella, Polaribacter and Flavobacterium, with one strain constituting a new bacterial genus in the family Flavobacteriaceae. After further phenotypic characterisation, this strain was given the name Olleya marilimosa, gen. nov., sp. nov. The ten strains were grown in batch culture and the EPS extracted, purified and partially characterized. Crude chemical, monosaccharide and molecular weight determinations showed that the EPS were diverse, even among closely related isolates. All EPS contained uronic acids to varying degrees and some also contained sulfate groups. Two EPS showed the presence of acetyl groups, with pyruvate ...