Proteomic and phenotypic approaches to assess nutritional physiology in Atlantic salmon ...

• Main Author: Nuez-Ortin, WG
• Format: Thesis
• Language: unknown
• Published: University Of Tasmania 2023
• Subjects: Atlantic salmon
• Online Access: https://dx.doi.org/10.25959/23239721; https://figshare.utas.edu.au/articles/thesis/Proteomic_and_phenotypic_approaches_to_assess_nutritional_physiology_in_Atlantic_salmon/23239721

Atlantic salmon aquaculture increasingly faces global sustainability and environmental challenges that influence fish growth, nutritional value of the final product and production efficiency. Proteomics has established itself as an exploratory approach to understand the impact of changes in environmental factors and to production strategies at a detailed biological level. This thesis aims to examine the use of label-free shotgun proteomics as a method to further our understanding of current and important key aspects of Atlantic salmon aquaculture production: triploidy, fish oil replacement and heat stress. Understanding diet- and environmentally-induced physiological changes in fish larvae is a major goal for the aquaculture industry. The application of proteomics to study larval fish is challenging due to the large dynamic range of protein expression in such complex biological matrix. Using sequential protein extraction to reduce sample complexity achieved proteome coverage of 40% greater than a ...