A molecular phylogenetic survey of polar sea ice microbial communities ...
The structure of the Antarctic sea ice cover is highly heterogenous, with composition in a given region depending on extremely localised physical processes. Past studies have revealed a wide range of sea ice microbial communities (SIMCO's), associated with a variety of ice types. Many of these...
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University Of Tasmania
2023
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ftdatacite:10.25959/23236505 2023-06-11T04:04:11+02:00 A molecular phylogenetic survey of polar sea ice microbial communities ... Brown, Mark Vincent 2023 https://dx.doi.org/10.25959/23236505 https://figshare.utas.edu.au/articles/thesis/A_molecular_phylogenetic_survey_of_polar_sea_ice_microbial_communities/23236505 unknown University Of Tasmania In Copyright http://rightsstatements.org/vocab/InC/1.0/ article-journal Text ScholarlyArticle Thesis 2023 ftdatacite https://doi.org/10.25959/23236505 2023-06-01T12:12:30Z The structure of the Antarctic sea ice cover is highly heterogenous, with composition in a given region depending on extremely localised physical processes. Past studies have revealed a wide range of sea ice microbial communities (SIMCO's), associated with a variety of ice types. Many of these assemblages are highly productive. Given the enormous extent of the ice cover these localised but highly productive communities constitute an important component of the regions biological processes. To date, our knowledge of the taxonomical composition of SIMCO's extends from culture based studies. This study employed culture independent molecular techniques to identify biodiversity in a variety of SIMCO's. Clone libraries of 16S rRNA genes were constructed from the total environmental DNA extracted from one Arctic and seven Antarctic sea ice samples using universally-conserved, Archaea-specific and Bacteria-specific 16S rDNA primers. A total of 539 recombinant clones were obtained. Restriction fragment length ... Thesis Antarc* Antarctic Arctic Sea ice DataCite Metadata Store (German National Library of Science and Technology) Antarctic Arctic The Antarctic |
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DataCite Metadata Store (German National Library of Science and Technology) |
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description |
The structure of the Antarctic sea ice cover is highly heterogenous, with composition in a given region depending on extremely localised physical processes. Past studies have revealed a wide range of sea ice microbial communities (SIMCO's), associated with a variety of ice types. Many of these assemblages are highly productive. Given the enormous extent of the ice cover these localised but highly productive communities constitute an important component of the regions biological processes. To date, our knowledge of the taxonomical composition of SIMCO's extends from culture based studies. This study employed culture independent molecular techniques to identify biodiversity in a variety of SIMCO's. Clone libraries of 16S rRNA genes were constructed from the total environmental DNA extracted from one Arctic and seven Antarctic sea ice samples using universally-conserved, Archaea-specific and Bacteria-specific 16S rDNA primers. A total of 539 recombinant clones were obtained. Restriction fragment length ... |
format |
Thesis |
author |
Brown, Mark Vincent |
spellingShingle |
Brown, Mark Vincent A molecular phylogenetic survey of polar sea ice microbial communities ... |
author_facet |
Brown, Mark Vincent |
author_sort |
Brown, Mark Vincent |
title |
A molecular phylogenetic survey of polar sea ice microbial communities ... |
title_short |
A molecular phylogenetic survey of polar sea ice microbial communities ... |
title_full |
A molecular phylogenetic survey of polar sea ice microbial communities ... |
title_fullStr |
A molecular phylogenetic survey of polar sea ice microbial communities ... |
title_full_unstemmed |
A molecular phylogenetic survey of polar sea ice microbial communities ... |
title_sort |
molecular phylogenetic survey of polar sea ice microbial communities ... |
publisher |
University Of Tasmania |
publishDate |
2023 |
url |
https://dx.doi.org/10.25959/23236505 https://figshare.utas.edu.au/articles/thesis/A_molecular_phylogenetic_survey_of_polar_sea_ice_microbial_communities/23236505 |
geographic |
Antarctic Arctic The Antarctic |
geographic_facet |
Antarctic Arctic The Antarctic |
genre |
Antarc* Antarctic Arctic Sea ice |
genre_facet |
Antarc* Antarctic Arctic Sea ice |
op_rights |
In Copyright http://rightsstatements.org/vocab/InC/1.0/ |
op_doi |
https://doi.org/10.25959/23236505 |
_version_ |
1768386068666646528 |