A molecular phylogenetic survey of polar sea ice microbial communities ...

• Main Author: Brown, Mark Vincent
• Format: Thesis
• Language: unknown
• Published: University Of Tasmania 2023
• Subjects: Antarctic; Arctic; The Antarctic; Antarc*; Sea ice
• Online Access: https://dx.doi.org/10.25959/23236505.v1; https://figshare.utas.edu.au/articles/thesis/A_molecular_phylogenetic_survey_of_polar_sea_ice_microbial_communities/23236505/1

The structure of the Antarctic sea ice cover is highly heterogenous, with composition in a given region depending on extremely localised physical processes. Past studies have revealed a wide range of sea ice microbial communities (SIMCO's), associated with a variety of ice types. Many of these assemblages are highly productive. Given the enormous extent of the ice cover these localised but highly productive communities constitute an important component of the regions biological processes. To date, our knowledge of the taxonomical composition of SIMCO's extends from culture based studies. This study employed culture independent molecular techniques to identify biodiversity in a variety of SIMCO's. Clone libraries of 16S rRNA genes were constructed from the total environmental DNA extracted from one Arctic and seven Antarctic sea ice samples using universally-conserved, Archaea-specific and Bacteria-specific 16S rDNA primers. A total of 539 recombinant clones were obtained. Restriction fragment length ...