Interactions between Neoparamoeba spp. and Atlantic salmon (Salmo salar L.) immune system components ...

• Main Author: Gross, Kally Anne
• Format: Thesis
• Language: unknown
• Published: University Of Tasmania 2023
• Subjects: Atlantic salmon; Salmo salar
• Online Access: https://dx.doi.org/10.25959/23233088; https://figshare.utas.edu.au/articles/thesis/Interactions_between_Neoparamoeba_spp_and_Atlantic_salmon_Salmo_salar_L_immune_system_components/23233088

The protozoan parasite Neoparamoeba spp. infects the gills of marine cultured Atlantic salmon in Tasmania, Australia causing amoebic gill disease (AGD) and if untreated causes significant production losses. Knowledge of the interaction between Neoparamoeba spp. and host immune factors or cells is limited; specific anti-Neoparamoeba spp. antibodies are present in the serum of fish naturally exposed and sheep anti-Neoparamoeba spp. antibodies do not affect the viability of Neoparamoeba spp. This thesis investigates in vitro and in vivo interactions between the host (Atlantic salmon) and Neoparamoeba spp. The effect of Neoparamoeba spp. on in vitro innate immune cell function was investigated. Sonicated Neoparamoeba spp. did not stimulate a respiratory burst response from anterior kidney leukocytes (P> 0.05). However, there was evidence of priming as cells incubated with the pathogen and then stimulated with phorbol myristate acetate (PMA) produced more (P < 0.05) reactive oxygen species (ROS) than cells ...