Gynogen induction and diploid restoration in the Pacific oyster Crassostrea gigas ...

• Main Author: Kent, GN
• Format: Thesis
• Language: unknown
• Published: University Of Tasmania 2023
• Subjects: Pacific; Crassostrea gigas; Pacific oyster
• Online Access: https://dx.doi.org/10.25959/23230787; https://figshare.utas.edu.au/articles/thesis/Gynogen_induction_and_diploid_restoration_in_the_Pacific_oyster_Crassostrea_gigas/23230787

A large proportion of stock improvement in agriculture and more recently aquaculture has come through breeding strategies that work to isolate and fix favourable characters and eliminate undesirable genetic qualities in the target species. The development of homozygous breeding lines, which allow fixing of desirable traits need to be progressed to enhance the effectiveness of breeding strategies. Such lines would also be a valuable research tool for examining genetic versus environmental influence on reproductive biology and stock performance. One potentially rapid and effective method that may produce 100% homozygous individuals is to create what are known as \double-haploids\" by suppressing the 1st cell cleavage in haploid zygotes. This project investigated the efficacy of haploid production in the Pacific oyster Crassostrea gigas by examining UV irradiation of the paternal DNA and restoration to the diploid state using the chemicals 6-dimethylaminopurine (6-DMAP) or Cytochalasin B (CB) to suppress either ...