Flavobacteria in the Southern Ocean ...
The abundance, spatial distribution and diversity of class Flavobacteria, a bacterial group with a major role in marine secondary production, was investigated in the Southern Ocean euphotic zone of the ice pack off Eastern Antarctica and along a transect at 140-144 degrees East between latitudes 44....
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University Of Tasmania
2023
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Online Access: | https://dx.doi.org/10.25959/23213915.v1 https://figshare.utas.edu.au/articles/thesis/Flavobacteria_in_the_Southern_Ocean/23213915/1 |
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ftdatacite:10.25959/23213915.v1 2023-06-11T04:07:13+02:00 Flavobacteria in the Southern Ocean ... Abell, GCJ 2023 https://dx.doi.org/10.25959/23213915.v1 https://figshare.utas.edu.au/articles/thesis/Flavobacteria_in_the_Southern_Ocean/23213915/1 unknown University Of Tasmania https://dx.doi.org/10.25959/23213915 In Copyright http://rightsstatements.org/vocab/InC/1.0/ article-journal Text ScholarlyArticle Thesis 2023 ftdatacite https://doi.org/10.25959/23213915.v110.25959/23213915 2023-06-01T12:09:52Z The abundance, spatial distribution and diversity of class Flavobacteria, a bacterial group with a major role in marine secondary production, was investigated in the Southern Ocean euphotic zone of the ice pack off Eastern Antarctica and along a transect at 140-144 degrees East between latitudes 44.7 degrees South to 63.5 degrees South. Samples were comparatively analysed using 16S rRNA gene-based denaturing gradient gel electrophoresis (DGGE), fluorescent in situ hybridisation, real-time PCR and sequence analysis. The results were subsequently compared with direct cultivation approaches. Surface seawater samples were filter-fractionated into particulate and planktonic fractions and the abundance of particle-associated Flavobacteria, ascertained with real-time PCR and DGGE band analysis using Flavobacteriaspecific primers. Flavobacteria abundance was found to be significantly higher in Polar Front Zone (PFZ) and Antarctic Zone (AZ) water samples compared to warmer, nutrient limited Temperate Zone (TZ) and ... Thesis Antarc* Antarctic Antarctica Southern Ocean DataCite Metadata Store (German National Library of Science and Technology) Antarctic Southern Ocean |
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DataCite Metadata Store (German National Library of Science and Technology) |
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description |
The abundance, spatial distribution and diversity of class Flavobacteria, a bacterial group with a major role in marine secondary production, was investigated in the Southern Ocean euphotic zone of the ice pack off Eastern Antarctica and along a transect at 140-144 degrees East between latitudes 44.7 degrees South to 63.5 degrees South. Samples were comparatively analysed using 16S rRNA gene-based denaturing gradient gel electrophoresis (DGGE), fluorescent in situ hybridisation, real-time PCR and sequence analysis. The results were subsequently compared with direct cultivation approaches. Surface seawater samples were filter-fractionated into particulate and planktonic fractions and the abundance of particle-associated Flavobacteria, ascertained with real-time PCR and DGGE band analysis using Flavobacteriaspecific primers. Flavobacteria abundance was found to be significantly higher in Polar Front Zone (PFZ) and Antarctic Zone (AZ) water samples compared to warmer, nutrient limited Temperate Zone (TZ) and ... |
format |
Thesis |
author |
Abell, GCJ |
spellingShingle |
Abell, GCJ Flavobacteria in the Southern Ocean ... |
author_facet |
Abell, GCJ |
author_sort |
Abell, GCJ |
title |
Flavobacteria in the Southern Ocean ... |
title_short |
Flavobacteria in the Southern Ocean ... |
title_full |
Flavobacteria in the Southern Ocean ... |
title_fullStr |
Flavobacteria in the Southern Ocean ... |
title_full_unstemmed |
Flavobacteria in the Southern Ocean ... |
title_sort |
flavobacteria in the southern ocean ... |
publisher |
University Of Tasmania |
publishDate |
2023 |
url |
https://dx.doi.org/10.25959/23213915.v1 https://figshare.utas.edu.au/articles/thesis/Flavobacteria_in_the_Southern_Ocean/23213915/1 |
geographic |
Antarctic Southern Ocean |
geographic_facet |
Antarctic Southern Ocean |
genre |
Antarc* Antarctic Antarctica Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctica Southern Ocean |
op_relation |
https://dx.doi.org/10.25959/23213915 |
op_rights |
In Copyright http://rightsstatements.org/vocab/InC/1.0/ |
op_doi |
https://doi.org/10.25959/23213915.v110.25959/23213915 |
_version_ |
1768380157229268992 |