Flavobacteria in the Southern Ocean ...

• Main Author: Abell, GCJ
• Format: Thesis
• Language: unknown
• Published: University Of Tasmania 2023
• Subjects: Antarctic; Southern Ocean; Antarc*; Antarctica
• Online Access: https://dx.doi.org/10.25959/23213915.v1; https://figshare.utas.edu.au/articles/thesis/Flavobacteria_in_the_Southern_Ocean/23213915/1

The abundance, spatial distribution and diversity of class Flavobacteria, a bacterial group with a major role in marine secondary production, was investigated in the Southern Ocean euphotic zone of the ice pack off Eastern Antarctica and along a transect at 140-144 degrees East between latitudes 44.7 degrees South to 63.5 degrees South. Samples were comparatively analysed using 16S rRNA gene-based denaturing gradient gel electrophoresis (DGGE), fluorescent in situ hybridisation, real-time PCR and sequence analysis. The results were subsequently compared with direct cultivation approaches. Surface seawater samples were filter-fractionated into particulate and planktonic fractions and the abundance of particle-associated Flavobacteria, ascertained with real-time PCR and DGGE band analysis using Flavobacteriaspecific primers. Flavobacteria abundance was found to be significantly higher in Polar Front Zone (PFZ) and Antarctic Zone (AZ) water samples compared to warmer, nutrient limited Temperate Zone (TZ) and ...