Detection of aquareovirus in farmed Tasmanian Atlantic salmon (Salmo salar) ...

• Main Author: Zainathan, SC
• Format: Thesis
• Language: unknown
• Published: University Of Tasmania 2023
• Subjects: Atlantic salmon; Salmo salar
• Online Access: https://dx.doi.org/10.25959/23206178.v1; https://figshare.utas.edu.au/articles/thesis/Detection_of_aquareovirus_in_farmed_Tasmanian_Atlantic_salmon_Salmo_salar_/23206178/1

This thesis focused on the detection and identification of Tasmanian Atlantic salmon aquareovirus (TSRV), which is one of the few viral agents of Atlantic salmon endemic in Tasmania. Due to the low pathogenicity and ubiquitous nature of TSRV, there has been little interest in the significance of this virus. However, more recently, TSRV infections appeared to be associated with diseased fish and concerns about the negative impact of infection with this virus on aquaculture productivity have increased. Industry's concerns regarding the significance of TSRV have resurfaced and recent research has indicated that under certain conditions TSRV could cause disease. Thus better management and control is being considered by industry and regulators. Validation of diagnostic methods has been a major focus in this thesis. Intra-laboratory and inter-laboratory comparison of PCR and virus isolation on piscine cell lines were carried out to determine the most sensitive diagnostic method, using tissues of farmed Atlantic ...