Prospects of using a consortium of hydrocarbon-oxidizing microorganisms for cleaning oil-polluted soil of the extreme north

• Main Authors: T. Yu. Korshunova, S. P. Chetverikov, O. N. Loginov
• Format: Dataset
• Language: Russian
• Published: Theoretical and Applied Ecology 2016
• Subjects: oil-polluted soil; consortium of microorganisms; biodestruction; heterotrophic ammonifying microorganisms; hydrocarbon-oxidizing microorganisms; khanty
• Online Access: https://dx.doi.org/10.25750/1995-4301-2016-1-095-097; http://envjournal.ru/ari/v2016/v1/files/16113.pdf

Khanty-Mansiysk autonomous okrug Ugra takes the first place in oil mining, it also has the biggest number of emergency situations connected with spills of oil and oil products. This paper shows the results of the field experiment of application of the natural consortium of microorganisms Acinetobacter sp. IB DT-5.1/1 and Ochrobactrum sp. IB DT-5.3/2 in cleaning oil-polluded soil in the region. The sample area (a wood-overgrown bog with a very high degree of oil-pollution – 70.3% (mas.)) was divided into allotments of 1 m2 . In September, 2013 each allotment was treated with 1 litre of bacterial suspension with the titre 1·108 cells/ml. Apart from the bacterial strain Pseudomonas sp. IB-1.1 capable of decomounding at a low positive temperature was used for comparing with. To assess the efficiece of the iopreparations we controlled the degree of carbon decomposing and the number of main phisological groups of microorganisms participating in oil products biotransformation (heterotrophic ammonifying and hydrocarbon-oxidizing). During 9 months of experiment at the allotments breated with the microorganisms consortium the number of residual hydrocarbons decreased to 40% (in mass) and the number of heterotrophic ammonifying microorganisms and hydrocarbon-oxidizing microorganisms grew by 2–3 orders of magnitude. The data obtained coincide with the results aquired at introduction of the psychrotolerant strain Pseudomonas sp. IB-1.1. At the control allotments without biopreparations introduction the number of heterotrophic ammonifying and hydrocarbon-oxidizing microorganisms did not change and the amount of oil products decreased slightly (to 67.1%). Due to high survival abitity the microorganisms consortium helps to clear oil pollution as well as to prolong the recultivation season by several months. The consortium of microorganisms Acinetobacter sp. IB DT-5.1/1 and Ochrobactrum sp. IB DT-5.3/2 is recommended as a basis of bioreparation for soil restoration in the zone of cold and moderate climate in autumt and early spring.