Stage-specific transcriptomic analyses of reproductive tissue in the Pacific oyster, Crassostrea gigas ...
The Pacific oyster is an important aquaculture species which suffers from reduced larval survival related to variation in state of gonad maturation among broodstock used for larval production. Gene expression was investigated as a potential indicator of reproductive stage. Stage-specific libraries w...
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ftdatacite:10.25549/usctheses-c3-365000 2024-03-31T07:52:23+00:00 Stage-specific transcriptomic analyses of reproductive tissue in the Pacific oyster, Crassostrea gigas ... Paul, Austin M. author 2014 https://dx.doi.org/10.25549/usctheses-c3-365000 https://digitallibrary.usc.edu/asset-management/2A3BF1LJ70WG en eng University of Southern California Digital Library (USC.DL) Biology degree program Doctor of Philosophy degree College of Letters, Arts and Sciences school thesis Dissertation Thesis 2014 ftdatacite https://doi.org/10.25549/usctheses-c3-365000 2024-03-04T12:42:22Z The Pacific oyster is an important aquaculture species which suffers from reduced larval survival related to variation in state of gonad maturation among broodstock used for larval production. Gene expression was investigated as a potential indicator of reproductive stage. Stage-specific libraries were created by RNA sequencing, which were used for testing and identification of stage-specific, differentially expressed genes. Multiple factors related to the normalization and interpretation of gene expression data were queried, including the role of deadenylation of mRNA in developing oocytes. A set of candidate biomarker genes were screened for their correlation with and ability to predict maturation stage in female oysters. The results justify further development and use of expression data to identify maturation state. ... Thesis Crassostrea gigas Pacific oyster DataCite Metadata Store (German National Library of Science and Technology) Pacific |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
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ftdatacite |
language |
English |
topic |
Biology degree program Doctor of Philosophy degree College of Letters, Arts and Sciences school |
spellingShingle |
Biology degree program Doctor of Philosophy degree College of Letters, Arts and Sciences school Paul, Austin M. author Stage-specific transcriptomic analyses of reproductive tissue in the Pacific oyster, Crassostrea gigas ... |
topic_facet |
Biology degree program Doctor of Philosophy degree College of Letters, Arts and Sciences school |
description |
The Pacific oyster is an important aquaculture species which suffers from reduced larval survival related to variation in state of gonad maturation among broodstock used for larval production. Gene expression was investigated as a potential indicator of reproductive stage. Stage-specific libraries were created by RNA sequencing, which were used for testing and identification of stage-specific, differentially expressed genes. Multiple factors related to the normalization and interpretation of gene expression data were queried, including the role of deadenylation of mRNA in developing oocytes. A set of candidate biomarker genes were screened for their correlation with and ability to predict maturation stage in female oysters. The results justify further development and use of expression data to identify maturation state. ... |
format |
Thesis |
author |
Paul, Austin M. author |
author_facet |
Paul, Austin M. author |
author_sort |
Paul, Austin M. author |
title |
Stage-specific transcriptomic analyses of reproductive tissue in the Pacific oyster, Crassostrea gigas ... |
title_short |
Stage-specific transcriptomic analyses of reproductive tissue in the Pacific oyster, Crassostrea gigas ... |
title_full |
Stage-specific transcriptomic analyses of reproductive tissue in the Pacific oyster, Crassostrea gigas ... |
title_fullStr |
Stage-specific transcriptomic analyses of reproductive tissue in the Pacific oyster, Crassostrea gigas ... |
title_full_unstemmed |
Stage-specific transcriptomic analyses of reproductive tissue in the Pacific oyster, Crassostrea gigas ... |
title_sort |
stage-specific transcriptomic analyses of reproductive tissue in the pacific oyster, crassostrea gigas ... |
publisher |
University of Southern California Digital Library (USC.DL) |
publishDate |
2014 |
url |
https://dx.doi.org/10.25549/usctheses-c3-365000 https://digitallibrary.usc.edu/asset-management/2A3BF1LJ70WG |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_doi |
https://doi.org/10.25549/usctheses-c3-365000 |
_version_ |
1795031492069425152 |