The antifungal activity of a thaumatin-like protein from oyster Crassostrea gigas
In the present study, a thaumatin-like protein (CgTLP) was identified from the oyster Crassostrea gigas. The full-length cDNA of CgTLP was of 913 bp with a 5’ untranslated regions (UTR) of 98 bp, a 3’ UTR of 80 bp, and an open reading frame (ORF) of 735 bp encoding a polypeptide of 244 residues. The...
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Invertebrate Survival Journal
2018
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ftdatacite:10.25431/1824-307x/isj.v15i1.210-222 2023-05-15T15:58:01+02:00 The antifungal activity of a thaumatin-like protein from oyster Crassostrea gigas Niu, X Xu, Q Wang, W Yu, Z Liu, Z Qu, C Liu, Y Gong, C Wang, L Song, L 2018 https://dx.doi.org/10.25431/1824-307x/isj.v15i1.210-222 http://www.isj.unimo.it/index.php/ISJ/article/view/427 en eng Invertebrate Survival Journal Text Article article-journal ScholarlyArticle 2018 ftdatacite https://doi.org/10.25431/1824-307x/isj.v15i1.210-222 2021-11-05T12:55:41Z In the present study, a thaumatin-like protein (CgTLP) was identified from the oyster Crassostrea gigas. The full-length cDNA of CgTLP was of 913 bp with a 5’ untranslated regions (UTR) of 98 bp, a 3’ UTR of 80 bp, and an open reading frame (ORF) of 735 bp encoding a polypeptide of 244 residues. The CgTLP gene was expressed ubiquitously in mantle, gonad, hemocytes, hepatopancreas, gill, and adductor muscle with the higher expression levels in adductor muscle, hemocytes, and hepatopancreas. Immunofluorescence assay indicated that CgTLP was mainly distributed in the cytoplasm of hemocytes. The mRNA expression levels of CgTLP in hemocytes were significantly up-regulated after the stimulations with mannan (13.69-fold, p < 0.05), Pichia pastoris (8.85-fold, p < 0.05) and polyinosinic-polycytidylic acid (3.62-fold, p < 0.05), but did not change significantly after stimulations with lipopolysaccharide, peptidoglycan, and Vibrio splendidus. The recombinant CgTLP protein (rCgTLP) significantly inhibited the proliferation of P. pastoris (p < 0.05), while no inhibition towards Staphylococcus aureus and V. splendidus. rCgTLP also displayed obvious β-1,3-glucanase activity, while no enzymatic activity towards chitin. These results collectively indicated that CgTLP was a homologue of TLP, which might play a vital role in defensing against fungal infection in C. gigas. : Invertebrate Survival Journal, Vol 15 No 1 (2018) Text Crassostrea gigas DataCite Metadata Store (German National Library of Science and Technology) |
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English |
description |
In the present study, a thaumatin-like protein (CgTLP) was identified from the oyster Crassostrea gigas. The full-length cDNA of CgTLP was of 913 bp with a 5’ untranslated regions (UTR) of 98 bp, a 3’ UTR of 80 bp, and an open reading frame (ORF) of 735 bp encoding a polypeptide of 244 residues. The CgTLP gene was expressed ubiquitously in mantle, gonad, hemocytes, hepatopancreas, gill, and adductor muscle with the higher expression levels in adductor muscle, hemocytes, and hepatopancreas. Immunofluorescence assay indicated that CgTLP was mainly distributed in the cytoplasm of hemocytes. The mRNA expression levels of CgTLP in hemocytes were significantly up-regulated after the stimulations with mannan (13.69-fold, p < 0.05), Pichia pastoris (8.85-fold, p < 0.05) and polyinosinic-polycytidylic acid (3.62-fold, p < 0.05), but did not change significantly after stimulations with lipopolysaccharide, peptidoglycan, and Vibrio splendidus. The recombinant CgTLP protein (rCgTLP) significantly inhibited the proliferation of P. pastoris (p < 0.05), while no inhibition towards Staphylococcus aureus and V. splendidus. rCgTLP also displayed obvious β-1,3-glucanase activity, while no enzymatic activity towards chitin. These results collectively indicated that CgTLP was a homologue of TLP, which might play a vital role in defensing against fungal infection in C. gigas. : Invertebrate Survival Journal, Vol 15 No 1 (2018) |
format |
Text |
author |
Niu, X Xu, Q Wang, W Yu, Z Liu, Z Qu, C Liu, Y Gong, C Wang, L Song, L |
spellingShingle |
Niu, X Xu, Q Wang, W Yu, Z Liu, Z Qu, C Liu, Y Gong, C Wang, L Song, L The antifungal activity of a thaumatin-like protein from oyster Crassostrea gigas |
author_facet |
Niu, X Xu, Q Wang, W Yu, Z Liu, Z Qu, C Liu, Y Gong, C Wang, L Song, L |
author_sort |
Niu, X |
title |
The antifungal activity of a thaumatin-like protein from oyster Crassostrea gigas |
title_short |
The antifungal activity of a thaumatin-like protein from oyster Crassostrea gigas |
title_full |
The antifungal activity of a thaumatin-like protein from oyster Crassostrea gigas |
title_fullStr |
The antifungal activity of a thaumatin-like protein from oyster Crassostrea gigas |
title_full_unstemmed |
The antifungal activity of a thaumatin-like protein from oyster Crassostrea gigas |
title_sort |
antifungal activity of a thaumatin-like protein from oyster crassostrea gigas |
publisher |
Invertebrate Survival Journal |
publishDate |
2018 |
url |
https://dx.doi.org/10.25431/1824-307x/isj.v15i1.210-222 http://www.isj.unimo.it/index.php/ISJ/article/view/427 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_doi |
https://doi.org/10.25431/1824-307x/isj.v15i1.210-222 |
_version_ |
1766393743683878912 |