Rapid differentiation of infectious salmon anemia virus avirulent (HPR0) from virulent (HPRΔ) variants using multiplex RT-qPCR ...
Infectious salmon anemia virus (ISAV; Isavirus salaris ) causes an economically important disease of Atlantic salmon ( Salmo salar L.). ISA outbreaks have resulted in significant losses of farmed salmon globally, often with a sudden onset. However, 2 phenotypically distinct variants of ISAV exist, e...
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ftdatacite:10.25384/sage.c.7023089.v1 2024-02-27T08:38:53+00:00 Rapid differentiation of infectious salmon anemia virus avirulent (HPR0) from virulent (HPRΔ) variants using multiplex RT-qPCR ... Rounsville, Thomas F. Polinski, Mark P. Marini, Alyssa G. Turner, Sarah M. Vendramin, Niccolò Cuenca, Argelia Pietrak, Michael R. Peterson, Brian C. Bouchard, Deborah A. 2024 https://dx.doi.org/10.25384/sage.c.7023089.v1 https://sage.figshare.com/collections/Rapid_differentiation_of_infectious_salmon_anemia_virus_avirulent_HPR0_from_virulent_HPR_variants_using_multiplex_RT-qPCR/7023089/1 unknown SAGE Journals https://dx.doi.org/10.25384/sage.c.7023089 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 Pharmacology and Pharmaceutical Sciences not elsewhere classified Veterinary Medicine Collection article 2024 ftdatacite https://doi.org/10.25384/sage.c.7023089.v110.25384/sage.c.7023089 2024-02-01T15:15:56Z Infectious salmon anemia virus (ISAV; Isavirus salaris ) causes an economically important disease of Atlantic salmon ( Salmo salar L.). ISA outbreaks have resulted in significant losses of farmed salmon globally, often with a sudden onset. However, 2 phenotypically distinct variants of ISAV exist, each with divergent disease outcomes, associated regulations, and control measures. ISAV-HPRΔ, also known as ISAV-HPR deleted, is responsible for ISA outbreaks; ISAV-HPR0, is avirulent and is not known to cause fish mortality. Current detection methodology requires genetic sequencing of ISAV-positive samples to differentiate phenotypes, which may slow responses to disease management. To increase the speed of phenotypic determinations of ISAV, we developed a new, rapid multiplex RT-qPCR method capable of 1) detecting if a sample contains any form of ISAV, 2) discriminating whether positive samples contain HPRΔ or HPR0, and 3) validating RNA extractions with an internal control, all in a single reaction. Following ... Article in Journal/Newspaper Atlantic salmon Salmo salar DataCite Metadata Store (German National Library of Science and Technology) |
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collection |
DataCite Metadata Store (German National Library of Science and Technology) |
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unknown |
topic |
Pharmacology and Pharmaceutical Sciences not elsewhere classified Veterinary Medicine |
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Pharmacology and Pharmaceutical Sciences not elsewhere classified Veterinary Medicine Rounsville, Thomas F. Polinski, Mark P. Marini, Alyssa G. Turner, Sarah M. Vendramin, Niccolò Cuenca, Argelia Pietrak, Michael R. Peterson, Brian C. Bouchard, Deborah A. Rapid differentiation of infectious salmon anemia virus avirulent (HPR0) from virulent (HPRΔ) variants using multiplex RT-qPCR ... |
topic_facet |
Pharmacology and Pharmaceutical Sciences not elsewhere classified Veterinary Medicine |
description |
Infectious salmon anemia virus (ISAV; Isavirus salaris ) causes an economically important disease of Atlantic salmon ( Salmo salar L.). ISA outbreaks have resulted in significant losses of farmed salmon globally, often with a sudden onset. However, 2 phenotypically distinct variants of ISAV exist, each with divergent disease outcomes, associated regulations, and control measures. ISAV-HPRΔ, also known as ISAV-HPR deleted, is responsible for ISA outbreaks; ISAV-HPR0, is avirulent and is not known to cause fish mortality. Current detection methodology requires genetic sequencing of ISAV-positive samples to differentiate phenotypes, which may slow responses to disease management. To increase the speed of phenotypic determinations of ISAV, we developed a new, rapid multiplex RT-qPCR method capable of 1) detecting if a sample contains any form of ISAV, 2) discriminating whether positive samples contain HPRΔ or HPR0, and 3) validating RNA extractions with an internal control, all in a single reaction. Following ... |
format |
Article in Journal/Newspaper |
author |
Rounsville, Thomas F. Polinski, Mark P. Marini, Alyssa G. Turner, Sarah M. Vendramin, Niccolò Cuenca, Argelia Pietrak, Michael R. Peterson, Brian C. Bouchard, Deborah A. |
author_facet |
Rounsville, Thomas F. Polinski, Mark P. Marini, Alyssa G. Turner, Sarah M. Vendramin, Niccolò Cuenca, Argelia Pietrak, Michael R. Peterson, Brian C. Bouchard, Deborah A. |
author_sort |
Rounsville, Thomas F. |
title |
Rapid differentiation of infectious salmon anemia virus avirulent (HPR0) from virulent (HPRΔ) variants using multiplex RT-qPCR ... |
title_short |
Rapid differentiation of infectious salmon anemia virus avirulent (HPR0) from virulent (HPRΔ) variants using multiplex RT-qPCR ... |
title_full |
Rapid differentiation of infectious salmon anemia virus avirulent (HPR0) from virulent (HPRΔ) variants using multiplex RT-qPCR ... |
title_fullStr |
Rapid differentiation of infectious salmon anemia virus avirulent (HPR0) from virulent (HPRΔ) variants using multiplex RT-qPCR ... |
title_full_unstemmed |
Rapid differentiation of infectious salmon anemia virus avirulent (HPR0) from virulent (HPRΔ) variants using multiplex RT-qPCR ... |
title_sort |
rapid differentiation of infectious salmon anemia virus avirulent (hpr0) from virulent (hprδ) variants using multiplex rt-qpcr ... |
publisher |
SAGE Journals |
publishDate |
2024 |
url |
https://dx.doi.org/10.25384/sage.c.7023089.v1 https://sage.figshare.com/collections/Rapid_differentiation_of_infectious_salmon_anemia_virus_avirulent_HPR0_from_virulent_HPR_variants_using_multiplex_RT-qPCR/7023089/1 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
https://dx.doi.org/10.25384/sage.c.7023089 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.25384/sage.c.7023089.v110.25384/sage.c.7023089 |
_version_ |
1792045869454852096 |