Rapid differentiation of infectious salmon anemia virus avirulent (HPR0) from virulent (HPRΔ) variants using multiplex RT-qPCR ...

• Main Authors: Rounsville, Thomas F., Polinski, Mark P., Marini, Alyssa G., Turner, Sarah M., Vendramin, Niccolò, Cuenca, Argelia, Pietrak, Michael R., Peterson, Brian C., Bouchard, Deborah A.
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: SAGE Journals 2024
• Subjects: Pharmacology and Pharmaceutical Sciences not elsewhere classified; Veterinary Medicine; Atlantic salmon; Salmo salar
• Online Access: https://dx.doi.org/10.25384/sage.c.7023089.v1; https://sage.figshare.com/collections/Rapid_differentiation_of_infectious_salmon_anemia_virus_avirulent_HPR0_from_virulent_HPR_variants_using_multiplex_RT-qPCR/7023089/1

Infectious salmon anemia virus (ISAV; Isavirus salaris ) causes an economically important disease of Atlantic salmon ( Salmo salar L.). ISA outbreaks have resulted in significant losses of farmed salmon globally, often with a sudden onset. However, 2 phenotypically distinct variants of ISAV exist, each with divergent disease outcomes, associated regulations, and control measures. ISAV-HPRΔ, also known as ISAV-HPR deleted, is responsible for ISA outbreaks; ISAV-HPR0, is avirulent and is not known to cause fish mortality. Current detection methodology requires genetic sequencing of ISAV-positive samples to differentiate phenotypes, which may slow responses to disease management. To increase the speed of phenotypic determinations of ISAV, we developed a new, rapid multiplex RT-qPCR method capable of 1) detecting if a sample contains any form of ISAV, 2) discriminating whether positive samples contain HPRΔ or HPR0, and 3) validating RNA extractions with an internal control, all in a single reaction. Following ...