Investigation of laboratory methods for characterization of aquatic viruses in fish infected experimentally with infectious salmon anemia virus ...

• Main Authors: Eckstrand, Chrissy D., Torrevillas, Brandi K., Wolking, Rebecca M., Bradway, Daniel S., Warg, Janet V., Clayton, Richard D., Williams, Laura B., Pessier, Allan P., Reno, Joetta Lynn, McMenamin-Snekvik, Kathleen M., Thompson, Jim, Baszler, Timothy, Snekvik, Kevin R.
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: SAGE Journals 2023
• Subjects: 111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified; FOS Clinical medicine; 70706 Veterinary Medicine; FOS Veterinary sciences; Atlantic salmon; Salmo salar
• Online Access: https://dx.doi.org/10.25384/sage.c.6655389.v1; https://sage.figshare.com/collections/Investigation_of_laboratory_methods_for_characterization_of_aquatic_viruses_in_fish_infected_experimentally_with_infectious_salmon_anemia_virus/6655389/1

Rapid growth in aquaculture has resulted in high-density production systems in ecologically and geographically novel conditions in which the emergence of diseases is inevitable. Well-characterized methods for detection and surveillance of infectious diseases are vital for rapid identification, response, and recovery to protect economic and food security. We implemented a proof-of-concept approach for virus detection using a known high-consequence fish pathogen, infectious salmon anemia virus (ISAV), as the archetypal pathogen. In fish infected with ISAV, we integrated histopathology, virus isolation, whole-genome sequencing (WGS), electron microscopy (EM), in situ hybridization (ISH), and reverse transcription real-time PCR (RT-rtPCR). Fresh-frozen and formalin-fixed tissues were collected from virus-infected, control, and sham-infected Atlantic salmon ( Salmo salar ). Microscopic differences were not evident between uninfected and infected fish. Viral cytopathic effect was observed in cell cultures ...