Investigation of the occurrence of Angiostrongylus vasorum in coyotes in southern Ontario, Canada
In North America, the only endemic focus for Angiostrongylus vasorum (French heartworm) was historically thought to occur in the southeastern part of the island of Newfoundland. However, reports of A. vasorum infection in wild canids in West Virginia, USA, and Nova Scotia, Canada, suggest the introd...
Main Authors: | , , , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
SAGE Journals
2021
|
Subjects: | |
Online Access: | https://dx.doi.org/10.25384/sage.c.5401376.v1 https://sage.figshare.com/collections/Investigation_of_the_occurrence_of_i_Angiostrongylus_vasorum_i_in_coyotes_in_southern_Ontario_Canada/5401376/1 |
id |
ftdatacite:10.25384/sage.c.5401376.v1 |
---|---|
record_format |
openpolar |
spelling |
ftdatacite:10.25384/sage.c.5401376.v1 2023-05-15T17:22:40+02:00 Investigation of the occurrence of Angiostrongylus vasorum in coyotes in southern Ontario, Canada Kotwa, Jonathon D. Schnyder, Manuela Jardine, Claire M. Deplazes, Peter Pearl, David L. Berke, Olaf Mercer, Nicola Peregrine, Andrew S. 2021 https://dx.doi.org/10.25384/sage.c.5401376.v1 https://sage.figshare.com/collections/Investigation_of_the_occurrence_of_i_Angiostrongylus_vasorum_i_in_coyotes_in_southern_Ontario_Canada/5401376/1 unknown SAGE Journals https://dx.doi.org/10.1177/10406387211011990 https://dx.doi.org/10.25384/sage.c.5401376 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY 111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified FOS Clinical medicine 70706 Veterinary Medicine FOS Veterinary sciences Collection article 2021 ftdatacite https://doi.org/10.25384/sage.c.5401376.v1 https://doi.org/10.1177/10406387211011990 https://doi.org/10.25384/sage.c.5401376 2021-11-05T12:55:41Z In North America, the only endemic focus for Angiostrongylus vasorum (French heartworm) was historically thought to occur in the southeastern part of the island of Newfoundland. However, reports of A. vasorum infection in wild canids in West Virginia, USA, and Nova Scotia, Canada, suggest the introduction of the parasite to mainland North America. We screened for A. vasorum in coyotes from across southern Ontario. Additionally, we evaluated the performance of ELISAs for detection of circulating A. vasorum antigen (Ag-ELISA) and antibodies against A. vasorum (Ab-ELISA) designed for use in sera or blood of foxes for use with coyotes in this region. Autopsies were performed on 397 coyotes, and lung tissue extract prepared from each carcass was tested via both ELISAs. The sensitivity and specificity for both tests were estimated in the absence of a gold standard using a 2-test single population Bayesian model; sensitivity and specificity priors were based on the performance of the assays in foxes in Switzerland. Eight coyotes tested positive for A. vasorum antigen; no animal was antibody positive. The estimated sensitivity and specificity of the Ag-ELISA were 90.8% (95% credible interval [CrI]: 83.8–95.6%) and 95.5% (95% CrI: 93.4–97.2%), respectively. For the Ab-ELISA, the estimated sensitivity and specificity were 41.9% (95% CrI: 32.1–51.9%) and 98.0% (95% CrI: 96.3–99.0%), respectively. Based on these findings and negative postmortem data for the same animals, there is insufficient evidence to suggest the presence of A. vasorum in southern Ontario coyotes. Article in Journal/Newspaper Newfoundland DataCite Metadata Store (German National Library of Science and Technology) Canada |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified FOS Clinical medicine 70706 Veterinary Medicine FOS Veterinary sciences |
spellingShingle |
111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified FOS Clinical medicine 70706 Veterinary Medicine FOS Veterinary sciences Kotwa, Jonathon D. Schnyder, Manuela Jardine, Claire M. Deplazes, Peter Pearl, David L. Berke, Olaf Mercer, Nicola Peregrine, Andrew S. Investigation of the occurrence of Angiostrongylus vasorum in coyotes in southern Ontario, Canada |
topic_facet |
111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified FOS Clinical medicine 70706 Veterinary Medicine FOS Veterinary sciences |
description |
In North America, the only endemic focus for Angiostrongylus vasorum (French heartworm) was historically thought to occur in the southeastern part of the island of Newfoundland. However, reports of A. vasorum infection in wild canids in West Virginia, USA, and Nova Scotia, Canada, suggest the introduction of the parasite to mainland North America. We screened for A. vasorum in coyotes from across southern Ontario. Additionally, we evaluated the performance of ELISAs for detection of circulating A. vasorum antigen (Ag-ELISA) and antibodies against A. vasorum (Ab-ELISA) designed for use in sera or blood of foxes for use with coyotes in this region. Autopsies were performed on 397 coyotes, and lung tissue extract prepared from each carcass was tested via both ELISAs. The sensitivity and specificity for both tests were estimated in the absence of a gold standard using a 2-test single population Bayesian model; sensitivity and specificity priors were based on the performance of the assays in foxes in Switzerland. Eight coyotes tested positive for A. vasorum antigen; no animal was antibody positive. The estimated sensitivity and specificity of the Ag-ELISA were 90.8% (95% credible interval [CrI]: 83.8–95.6%) and 95.5% (95% CrI: 93.4–97.2%), respectively. For the Ab-ELISA, the estimated sensitivity and specificity were 41.9% (95% CrI: 32.1–51.9%) and 98.0% (95% CrI: 96.3–99.0%), respectively. Based on these findings and negative postmortem data for the same animals, there is insufficient evidence to suggest the presence of A. vasorum in southern Ontario coyotes. |
format |
Article in Journal/Newspaper |
author |
Kotwa, Jonathon D. Schnyder, Manuela Jardine, Claire M. Deplazes, Peter Pearl, David L. Berke, Olaf Mercer, Nicola Peregrine, Andrew S. |
author_facet |
Kotwa, Jonathon D. Schnyder, Manuela Jardine, Claire M. Deplazes, Peter Pearl, David L. Berke, Olaf Mercer, Nicola Peregrine, Andrew S. |
author_sort |
Kotwa, Jonathon D. |
title |
Investigation of the occurrence of Angiostrongylus vasorum in coyotes in southern Ontario, Canada |
title_short |
Investigation of the occurrence of Angiostrongylus vasorum in coyotes in southern Ontario, Canada |
title_full |
Investigation of the occurrence of Angiostrongylus vasorum in coyotes in southern Ontario, Canada |
title_fullStr |
Investigation of the occurrence of Angiostrongylus vasorum in coyotes in southern Ontario, Canada |
title_full_unstemmed |
Investigation of the occurrence of Angiostrongylus vasorum in coyotes in southern Ontario, Canada |
title_sort |
investigation of the occurrence of angiostrongylus vasorum in coyotes in southern ontario, canada |
publisher |
SAGE Journals |
publishDate |
2021 |
url |
https://dx.doi.org/10.25384/sage.c.5401376.v1 https://sage.figshare.com/collections/Investigation_of_the_occurrence_of_i_Angiostrongylus_vasorum_i_in_coyotes_in_southern_Ontario_Canada/5401376/1 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Newfoundland |
genre_facet |
Newfoundland |
op_relation |
https://dx.doi.org/10.1177/10406387211011990 https://dx.doi.org/10.25384/sage.c.5401376 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.25384/sage.c.5401376.v1 https://doi.org/10.1177/10406387211011990 https://doi.org/10.25384/sage.c.5401376 |
_version_ |
1766109472893173760 |