Necrotizing Ventriculitis in Fledgling Chimney Swifts ( Chaetura Pelagica ) Associated With a Novel Adenovirus, Chimney Swift Adenovirus-1 (CsAdV-1)
Five chimney swift fledglings died following a progressive loss of appetite and condition while being cared for by an experienced wildlife rehabilitator. All animals had severe necrotizing and heterophilic ventriculitis, with myriad epithelial cells characterized by karyomegaly with intranuclear inc...
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ftdatacite:10.25384/sage.c.4585928.v1 2023-05-15T13:49:59+02:00 Necrotizing Ventriculitis in Fledgling Chimney Swifts ( Chaetura Pelagica ) Associated With a Novel Adenovirus, Chimney Swift Adenovirus-1 (CsAdV-1) Needle, David B. Wise, Annabel G. Gregory, Christopher R. Maes, Roger K. Sidor, Inga F. Branson W. Ritchie Dalen Agnew 2019 https://dx.doi.org/10.25384/sage.c.4585928.v1 https://sage.figshare.com/collections/Necrotizing_Ventriculitis_in_Fledgling_Chimney_Swifts_i_Chaetura_Pelagica_i_Associated_With_a_Novel_Adenovirus_Chimney_Swift_Adenovirus-1_CsAdV-1_/4585928/1 unknown Figshare https://dx.doi.org/10.1177/0300985819861717 https://dx.doi.org/10.25384/sage.c.4585928 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY 111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified FOS Clinical medicine 70706 Veterinary Medicine FOS Veterinary sciences Collection article 2019 ftdatacite https://doi.org/10.25384/sage.c.4585928.v1 https://doi.org/10.1177/0300985819861717 https://doi.org/10.25384/sage.c.4585928 2021-11-05T12:55:41Z Five chimney swift fledglings died following a progressive loss of appetite and condition while being cared for by an experienced wildlife rehabilitator. All animals had severe necrotizing and heterophilic ventriculitis, with myriad epithelial cells characterized by karyomegaly with intranuclear inclusion bodies. Transmission electron microscopy showed distention of epithelial cell nuclei and chromatin peripheralization by nonenveloped, icosahedral, 75- to 85-nm-diameter virions. Degenerate nested PCR for a highly conserved region of the adenovirus DNA polymerase gene was positive. BLAST analysis of the amplicon sequence indicated the presence of a novel adenovirus, with 74% homology to Antarctic penguin adenoviruses and 72% homology to a bat adenovirus, at low query coverages of only 65% and 63%, respectively. BLAST analysis of the predicted amino acid sequence generated the highest scores for squamate adenoviruses at 100% query coverage. Based on phylogenetic analysis of the partial amino acid sequence of the DNA polymerase, the chimney swift virus was a novel adenovirus most closely related to the Atadenovirus genus. Using a probe based on the novel viral sequence, DNA in situ hybridization identified viral nucleic acid in the nucleus. While the tentatively named chimney swift adenovirus-1 (CsAdV-1) is so far classified with the Atadenoviruses, it is relatively divergent from other members of that genus and may represent the first identified member of a new genus of Adenoviruses. Article in Journal/Newspaper Antarc* Antarctic DataCite Metadata Store (German National Library of Science and Technology) Antarctic The Chimney ENVELOPE(-55.748,-55.748,52.617,52.617) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified FOS Clinical medicine 70706 Veterinary Medicine FOS Veterinary sciences |
spellingShingle |
111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified FOS Clinical medicine 70706 Veterinary Medicine FOS Veterinary sciences Needle, David B. Wise, Annabel G. Gregory, Christopher R. Maes, Roger K. Sidor, Inga F. Branson W. Ritchie Dalen Agnew Necrotizing Ventriculitis in Fledgling Chimney Swifts ( Chaetura Pelagica ) Associated With a Novel Adenovirus, Chimney Swift Adenovirus-1 (CsAdV-1) |
topic_facet |
111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified FOS Clinical medicine 70706 Veterinary Medicine FOS Veterinary sciences |
description |
Five chimney swift fledglings died following a progressive loss of appetite and condition while being cared for by an experienced wildlife rehabilitator. All animals had severe necrotizing and heterophilic ventriculitis, with myriad epithelial cells characterized by karyomegaly with intranuclear inclusion bodies. Transmission electron microscopy showed distention of epithelial cell nuclei and chromatin peripheralization by nonenveloped, icosahedral, 75- to 85-nm-diameter virions. Degenerate nested PCR for a highly conserved region of the adenovirus DNA polymerase gene was positive. BLAST analysis of the amplicon sequence indicated the presence of a novel adenovirus, with 74% homology to Antarctic penguin adenoviruses and 72% homology to a bat adenovirus, at low query coverages of only 65% and 63%, respectively. BLAST analysis of the predicted amino acid sequence generated the highest scores for squamate adenoviruses at 100% query coverage. Based on phylogenetic analysis of the partial amino acid sequence of the DNA polymerase, the chimney swift virus was a novel adenovirus most closely related to the Atadenovirus genus. Using a probe based on the novel viral sequence, DNA in situ hybridization identified viral nucleic acid in the nucleus. While the tentatively named chimney swift adenovirus-1 (CsAdV-1) is so far classified with the Atadenoviruses, it is relatively divergent from other members of that genus and may represent the first identified member of a new genus of Adenoviruses. |
format |
Article in Journal/Newspaper |
author |
Needle, David B. Wise, Annabel G. Gregory, Christopher R. Maes, Roger K. Sidor, Inga F. Branson W. Ritchie Dalen Agnew |
author_facet |
Needle, David B. Wise, Annabel G. Gregory, Christopher R. Maes, Roger K. Sidor, Inga F. Branson W. Ritchie Dalen Agnew |
author_sort |
Needle, David B. |
title |
Necrotizing Ventriculitis in Fledgling Chimney Swifts ( Chaetura Pelagica ) Associated With a Novel Adenovirus, Chimney Swift Adenovirus-1 (CsAdV-1) |
title_short |
Necrotizing Ventriculitis in Fledgling Chimney Swifts ( Chaetura Pelagica ) Associated With a Novel Adenovirus, Chimney Swift Adenovirus-1 (CsAdV-1) |
title_full |
Necrotizing Ventriculitis in Fledgling Chimney Swifts ( Chaetura Pelagica ) Associated With a Novel Adenovirus, Chimney Swift Adenovirus-1 (CsAdV-1) |
title_fullStr |
Necrotizing Ventriculitis in Fledgling Chimney Swifts ( Chaetura Pelagica ) Associated With a Novel Adenovirus, Chimney Swift Adenovirus-1 (CsAdV-1) |
title_full_unstemmed |
Necrotizing Ventriculitis in Fledgling Chimney Swifts ( Chaetura Pelagica ) Associated With a Novel Adenovirus, Chimney Swift Adenovirus-1 (CsAdV-1) |
title_sort |
necrotizing ventriculitis in fledgling chimney swifts ( chaetura pelagica ) associated with a novel adenovirus, chimney swift adenovirus-1 (csadv-1) |
publisher |
Figshare |
publishDate |
2019 |
url |
https://dx.doi.org/10.25384/sage.c.4585928.v1 https://sage.figshare.com/collections/Necrotizing_Ventriculitis_in_Fledgling_Chimney_Swifts_i_Chaetura_Pelagica_i_Associated_With_a_Novel_Adenovirus_Chimney_Swift_Adenovirus-1_CsAdV-1_/4585928/1 |
long_lat |
ENVELOPE(-55.748,-55.748,52.617,52.617) |
geographic |
Antarctic The Chimney |
geographic_facet |
Antarctic The Chimney |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
https://dx.doi.org/10.1177/0300985819861717 https://dx.doi.org/10.25384/sage.c.4585928 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.25384/sage.c.4585928.v1 https://doi.org/10.1177/0300985819861717 https://doi.org/10.25384/sage.c.4585928 |
_version_ |
1766252678738870272 |