MassIVE MSV000085868 - Physiological and molecular responses to ocean acidification in a model marine symbiosis : Pocilloporid corals (Pocillopora acuta) from an upwelling coral reef in Southern Taiwan (Houbihu) were exposed to one of four experimental treatments: ambient temperature (25C)+ambient pCO2 (~430 ppm), ambient temperature+high pCO2 (~850 ppm), high temperature (29C)+ambient pCO2, or high temperature+high pCO2. Proteins were extracted from 2-3 replicates per treatment (n=11 total), digested, and analyzed by iTRAQ followed by nano-liquid chromatography and MS/MS. Since there are only eight iTRAQ labels, 6 and 5 samples were run in each batch ("1" & "2" in the attached documents), and a normalizer sample was labeled with iTRAQ tag 113 and run in both batches to control for batch-to-batch variation. I am attaching the RAW files, MZML files, MZID files, the P. acuta-Cladocopium transcriptome (conceptually translated to proteins; as a fasta file), a supplemental data (Excel) file, and the associated manuscript, which has not yet been submitted and it subject to change.
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MassIVE
2020
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| Online Access: | https://dx.doi.org/10.25345/c5546q https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?accession=MSV000085868 |
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ftdatacite:10.25345/c5546q 2023-05-15T17:51:09+02:00 MassIVE MSV000085868 - Physiological and molecular responses to ocean acidification in a model marine symbiosis : Pocilloporid corals (Pocillopora acuta) from an upwelling coral reef in Southern Taiwan (Houbihu) were exposed to one of four experimental treatments: ambient temperature (25C)+ambient pCO2 (~430 ppm), ambient temperature+high pCO2 (~850 ppm), high temperature (29C)+ambient pCO2, or high temperature+high pCO2. Proteins were extracted from 2-3 replicates per treatment (n=11 total), digested, and analyzed by iTRAQ followed by nano-liquid chromatography and MS/MS. Since there are only eight iTRAQ labels, 6 and 5 samples were run in each batch ("1" & "2" in the attached documents), and a normalizer sample was labeled with iTRAQ tag 113 and run in both batches to control for batch-to-batch variation. I am attaching the RAW files, MZML files, MZID files, the P. acuta-Cladocopium transcriptome (conceptually translated to proteins; as a fasta file), a supplemental data (Excel) file, and the associated manuscript, which has not yet been submitted and it subject to change. Mayfield, Anderson 2020 https://dx.doi.org/10.25345/c5546q https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?accession=MSV000085868 en eng MassIVE Mass Spectrometry dataset Dataset 2020 ftdatacite https://doi.org/10.25345/c5546q 2021-11-05T12:55:41Z Dataset Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
| collection |
DataCite Metadata Store (German National Library of Science and Technology) |
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ftdatacite |
| language |
English |
| format |
Dataset |
| author |
Mayfield, Anderson |
| spellingShingle |
Mayfield, Anderson MassIVE MSV000085868 - Physiological and molecular responses to ocean acidification in a model marine symbiosis : Pocilloporid corals (Pocillopora acuta) from an upwelling coral reef in Southern Taiwan (Houbihu) were exposed to one of four experimental treatments: ambient temperature (25C)+ambient pCO2 (~430 ppm), ambient temperature+high pCO2 (~850 ppm), high temperature (29C)+ambient pCO2, or high temperature+high pCO2. Proteins were extracted from 2-3 replicates per treatment (n=11 total), digested, and analyzed by iTRAQ followed by nano-liquid chromatography and MS/MS. Since there are only eight iTRAQ labels, 6 and 5 samples were run in each batch ("1" & "2" in the attached documents), and a normalizer sample was labeled with iTRAQ tag 113 and run in both batches to control for batch-to-batch variation. I am attaching the RAW files, MZML files, MZID files, the P. acuta-Cladocopium transcriptome (conceptually translated to proteins; as a fasta file), a supplemental data (Excel) file, and the associated manuscript, which has not yet been submitted and it subject to change. |
| author_facet |
Mayfield, Anderson |
| author_sort |
Mayfield, Anderson |
| title |
MassIVE MSV000085868 - Physiological and molecular responses to ocean acidification in a model marine symbiosis : Pocilloporid corals (Pocillopora acuta) from an upwelling coral reef in Southern Taiwan (Houbihu) were exposed to one of four experimental treatments: ambient temperature (25C)+ambient pCO2 (~430 ppm), ambient temperature+high pCO2 (~850 ppm), high temperature (29C)+ambient pCO2, or high temperature+high pCO2. Proteins were extracted from 2-3 replicates per treatment (n=11 total), digested, and analyzed by iTRAQ followed by nano-liquid chromatography and MS/MS. Since there are only eight iTRAQ labels, 6 and 5 samples were run in each batch ("1" & "2" in the attached documents), and a normalizer sample was labeled with iTRAQ tag 113 and run in both batches to control for batch-to-batch variation. I am attaching the RAW files, MZML files, MZID files, the P. acuta-Cladocopium transcriptome (conceptually translated to proteins; as a fasta file), a supplemental data (Excel) file, and the associated manuscript, which has not yet been submitted and it subject to change. |
| title_short |
MassIVE MSV000085868 - Physiological and molecular responses to ocean acidification in a model marine symbiosis : Pocilloporid corals (Pocillopora acuta) from an upwelling coral reef in Southern Taiwan (Houbihu) were exposed to one of four experimental treatments: ambient temperature (25C)+ambient pCO2 (~430 ppm), ambient temperature+high pCO2 (~850 ppm), high temperature (29C)+ambient pCO2, or high temperature+high pCO2. Proteins were extracted from 2-3 replicates per treatment (n=11 total), digested, and analyzed by iTRAQ followed by nano-liquid chromatography and MS/MS. Since there are only eight iTRAQ labels, 6 and 5 samples were run in each batch ("1" & "2" in the attached documents), and a normalizer sample was labeled with iTRAQ tag 113 and run in both batches to control for batch-to-batch variation. I am attaching the RAW files, MZML files, MZID files, the P. acuta-Cladocopium transcriptome (conceptually translated to proteins; as a fasta file), a supplemental data (Excel) file, and the associated manuscript, which has not yet been submitted and it subject to change. |
| title_full |
MassIVE MSV000085868 - Physiological and molecular responses to ocean acidification in a model marine symbiosis : Pocilloporid corals (Pocillopora acuta) from an upwelling coral reef in Southern Taiwan (Houbihu) were exposed to one of four experimental treatments: ambient temperature (25C)+ambient pCO2 (~430 ppm), ambient temperature+high pCO2 (~850 ppm), high temperature (29C)+ambient pCO2, or high temperature+high pCO2. Proteins were extracted from 2-3 replicates per treatment (n=11 total), digested, and analyzed by iTRAQ followed by nano-liquid chromatography and MS/MS. Since there are only eight iTRAQ labels, 6 and 5 samples were run in each batch ("1" & "2" in the attached documents), and a normalizer sample was labeled with iTRAQ tag 113 and run in both batches to control for batch-to-batch variation. I am attaching the RAW files, MZML files, MZID files, the P. acuta-Cladocopium transcriptome (conceptually translated to proteins; as a fasta file), a supplemental data (Excel) file, and the associated manuscript, which has not yet been submitted and it subject to change. |
| title_fullStr |
MassIVE MSV000085868 - Physiological and molecular responses to ocean acidification in a model marine symbiosis : Pocilloporid corals (Pocillopora acuta) from an upwelling coral reef in Southern Taiwan (Houbihu) were exposed to one of four experimental treatments: ambient temperature (25C)+ambient pCO2 (~430 ppm), ambient temperature+high pCO2 (~850 ppm), high temperature (29C)+ambient pCO2, or high temperature+high pCO2. Proteins were extracted from 2-3 replicates per treatment (n=11 total), digested, and analyzed by iTRAQ followed by nano-liquid chromatography and MS/MS. Since there are only eight iTRAQ labels, 6 and 5 samples were run in each batch ("1" & "2" in the attached documents), and a normalizer sample was labeled with iTRAQ tag 113 and run in both batches to control for batch-to-batch variation. I am attaching the RAW files, MZML files, MZID files, the P. acuta-Cladocopium transcriptome (conceptually translated to proteins; as a fasta file), a supplemental data (Excel) file, and the associated manuscript, which has not yet been submitted and it subject to change. |
| title_full_unstemmed |
MassIVE MSV000085868 - Physiological and molecular responses to ocean acidification in a model marine symbiosis : Pocilloporid corals (Pocillopora acuta) from an upwelling coral reef in Southern Taiwan (Houbihu) were exposed to one of four experimental treatments: ambient temperature (25C)+ambient pCO2 (~430 ppm), ambient temperature+high pCO2 (~850 ppm), high temperature (29C)+ambient pCO2, or high temperature+high pCO2. Proteins were extracted from 2-3 replicates per treatment (n=11 total), digested, and analyzed by iTRAQ followed by nano-liquid chromatography and MS/MS. Since there are only eight iTRAQ labels, 6 and 5 samples were run in each batch ("1" & "2" in the attached documents), and a normalizer sample was labeled with iTRAQ tag 113 and run in both batches to control for batch-to-batch variation. I am attaching the RAW files, MZML files, MZID files, the P. acuta-Cladocopium transcriptome (conceptually translated to proteins; as a fasta file), a supplemental data (Excel) file, and the associated manuscript, which has not yet been submitted and it subject to change. |
| title_sort |
massive msv000085868 - physiological and molecular responses to ocean acidification in a model marine symbiosis : pocilloporid corals (pocillopora acuta) from an upwelling coral reef in southern taiwan (houbihu) were exposed to one of four experimental treatments: ambient temperature (25c)+ambient pco2 (~430 ppm), ambient temperature+high pco2 (~850 ppm), high temperature (29c)+ambient pco2, or high temperature+high pco2. proteins were extracted from 2-3 replicates per treatment (n=11 total), digested, and analyzed by itraq followed by nano-liquid chromatography and ms/ms. since there are only eight itraq labels, 6 and 5 samples were run in each batch ("1" & "2" in the attached documents), and a normalizer sample was labeled with itraq tag 113 and run in both batches to control for batch-to-batch variation. i am attaching the raw files, mzml files, mzid files, the p. acuta-cladocopium transcriptome (conceptually translated to proteins; as a fasta file), a supplemental data (excel) file, and the associated manuscript, which has not yet been submitted and it subject to change. |
| publisher |
MassIVE |
| publishDate |
2020 |
| url |
https://dx.doi.org/10.25345/c5546q https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?accession=MSV000085868 |
| genre |
Ocean acidification |
| genre_facet |
Ocean acidification |
| op_doi |
https://doi.org/10.25345/c5546q |
| _version_ |
1766158211781492736 |